Detail of fig4D_bioengineeredhair_day23



Project
Title
Macromorphological image of the anagen phase of the hair cycles in mouse iPS cell-derived bioengineered hair at day23
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
8.4 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
iPS
Cell Line
-

Datatype
hair regeneration
Molecular Function (MF)
Biological Process (BP)
organogenesis ( GO:0048513 ) hair follicle development ( GO:0001943 ) hair cycle ( GO:0042633 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 3.6025 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
BrightfieldMicroscope
Acquisition mode
BrightField
Contrast method
Darkfield
Microscope model
SteREO Lumar.V12, AXIOcAM (Carl Zeiss)
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Takagi et al. (2016) Science Advances, 2(4): e1500887.
Related paper(s)

Ryoji Takagi, Junko Ishimaru, Ayaka Sugawara, Koh-Ei Toyoshima, Kentaro Ishida, Miho Ogawa, Kei Sakakibara, Kyosuke Asakawa, Akitoshi Kashiwakura, Masamitsu Oshima, Ryohei Minamide, Akio Sato, Toshihiro Yoshitake, Akira Takeda, Hiroshi Egusa, Takashi Tsuji (2016) Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model., Science advances, Volume 2, Number 4, pp. e1500887

Published in 2016 Apr (Electronic publication in April 1, 2016, midnight )

(Abstract) The integumentary organ system is a complex system that plays important roles in waterproofing, cushioning, protecting deeper tissues, excreting waste, and thermoregulation. We developed a novel in vivo transplantation model designated as a clustering-dependent embryoid body transplantation method and generated a bioengineered three-dimensional (3D) integumentary organ system, including appendage organs such as hair follicles and sebaceous glands, from induced pluripotent stem cells. This bioengineered 3D integumentary organ system was fully functional following transplantation into nude mice and could be properly connected to surrounding host tissues, such as the epidermis, arrector pili muscles, and nerve fibers, without tumorigenesis. The bioengineered hair follicles in the 3D integumentary organ system also showed proper hair eruption and hair cycles, including the rearrangement of follicular stem cells and their niches. Potential applications of the 3D integumentary organ system include an in vitro assay system, an animal model alternative, and a bioengineered organ replacement therapy.
(MeSH Terms)

Contact
Takashi Tsuji , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Organ Regeneration
Contributors
Ryoji Takagi, Junko Ishimaru, Ayaka Sugawara, Koh-ei Toyoshima, Kentaro Ishida, Miho Ogawa, Kei Sakakibara, Kyosuke Asakawa, Akitoshi Kashiwakura, Masamitsu Oshima, Ryohei Minamide, Akio Sato, Toshihiro Yoshitake, Akira Takeda, Hiroshi Egusa, Takashi Tsuji

OMERO Dataset
OMERO Project
Source