Detail of fig1d_3min

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Project
Title
Time lapse images of pupal molt of the Japanese rhinoceros beetle Trypoxylus dichotomus at different time stage
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
1.6 MB

Organism
Trypoxylus dichotomus ( NCBI:txid273928 )
Strain(s)
-
Cell Line
-

Datatype
horn primordia dynamic
Molecular Function (MF)
Biological Process (BP)
cell migration ( GO:0016477 ) metamorphosis ( GO:0007552 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
TimeLapse
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Matsuda et al. (2017) Sci Rep, 7(1): 13939.
Related paper(s)

Keisuke Matsuda, Hiroki Gotoh, Yuki Tajika, Takamichi Sushida, Hitoshi Aonuma, Teruyuki Niimi, Masakazu Akiyama, Yasuhiro Inoue, Shigeru Kondo (2017) Complex furrows in a 2D epithelial sheet code the 3D structure of a beetle horn., Scientific reports, Volume 7, Number 1, pp. 13939

Published in 2017 Oct 24 (Electronic publication in Oct. 24, 2017, midnight )

(Abstract) The external organs of holometabolous insects are generated through two consecutive processes: the development of imaginal primordia and their subsequent transformation into the adult structures. During the latter process, many different phenomena at the cellular level (e.g. cell shape changes, cell migration, folding and unfolding of epithelial sheets) contribute to the drastic changes observed in size and shape. Because of this complexity, the logic behind the formation of the 3D structure of adult external organs remains largely unknown. In this report, we investigated the metamorphosis of the horn in the Japanese rhinoceros beetle Trypoxylus dichotomus. The horn primordia is essentially a 2D epithelial cell sheet with dense furrows. We experimentally unfolded these furrows using three different methods and found that the furrow pattern solely determines the 3D horn structure, indicating that horn formation in beetles occurs by two distinct processes: formation of the furrows and subsequently unfolding them. We postulate that this developmental simplicity offers an inherent advantage to understanding the principles that guide 3D morphogenesis in insects.
(MeSH Terms)

Contact
Hiroki Gotoh , Nagoya University , Graduate School of Bioagricultural Sciences
Contributors
Keisuke Matsuda, Hiroki Gotoh, Yuki Tajika, Takamichi Sushida, Hitoshi Aonuma, Teruyuki Niimi, Masakazu Akiyama, Yasuhiro Inoue, Shigeru Kondo

OMERO Dataset
OMERO Project
Source