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Key
Organism
Strain
Cell Line
Gene Symbol
Protein name
Protein tag
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Gene Ontology
Biological Imaging Methods
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Detail of fig5e_wt_day2
SSBD:OMERO.gallery Dataset
SSBD:OMERO Dataset
SSBD:OMERO.gallery Project
SSBD:OMERO Project
4D View for Quantitative Data
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SSBD:downloads
Project
89-Isshiki-SpineDyn
SSBD:Repository
ssbd-repos-000089
Title
In vivo time-lapse image of gephyrin-GFP and dendrites filled with DsRed2 in the SSC of wild-type mice over 2 days.
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
33.0 MB
Organism
Mus musculus
(
NCBI:txid10090
)
Strain(s)
-
Cell Line
-
Protein names
gephyrin
Protein tags
GFP, DsRed2
Datatype
spine dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
spine synapse (
GO:0106033
) , postsynaptic density (
GO:0014069
)
Biological Imaging Method
XYZ Scale
XY: 0.1534 micrometer/pixel, Z: 0.75 micrometer/slice
T scale
2 day for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FV-300
Detector model
-
Objective model
XLPlan N, numerical aperture 1.05, Olympus
Filter set
-
Summary of Methods
See details in Isshiki et al. (2014) Nat Commun, 5: 4742.
Related paper(s)
Contact
Shigeo Okabe , University of Tokyo , Department of Cellular Neurobiology, Graduate School of Medicine
Contributors
Masaaki Isshiki, Shinji Tanaka, Toshihiko Kuriu, Katsuhiko Tabuchi, Toru Takumi, Shigeo Okabe
OMERO Dataset
https://ssbd.riken.jp/omero/webclient/?show=dataset-1232
OMERO Project
https://ssbd.riken.jp/omero/webclient/?show=project-201
Source
https://ssbd.riken.jp/data/89-Isshiki-SpineDyn/source/fig5e_wt_day2.zip
(15.0 MB)