Detail of Fig4H_csTreh_E3rd_ST4hr



Project
Title
Immunostaining image of the autophagy in theTresh mutant drosophila fed after 4 hours starved.
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
1.5 MB

Organism
D. melanogaster ( NCBI:txid7227 )
Strain(s)
-
Cell Line
-

Datatype
LysoTracker-positive vesicle, nucleus
Molecular Function (MF)
Biological Process (BP)
autophagy ( GO:0006914 )
Cellular Component (CC)
nucleus ( GO:0005634 )
Biological Imaging Method
XYZ Scale
XY: 0.156 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Zeiss LSM700
Detector model
PMT
Objective model
C-Apochromat 40x/1.20 W Korr M27
Filter set
-

Summary of Methods
See details in Yasugi et al. (2017) Sci Rep, 7(1): 1619.
Related paper(s)

Tetsuo Yasugi, Takayuki Yamada, Takashi Nishimura (2017) Adaptation to dietary conditions by trehalose metabolism in Drosophila., Scientific reports, Volume 7, Number 1, pp. 1619

Published in 2017 May 9 (Electronic publication in May 9, 2017, midnight )

(Abstract) Trehalose is a non-reducing disaccharide that serves as the main sugar component of haemolymph in insects. Trehalose hydrolysis enzyme, called trehalase, is highly conserved from bacteria to humans. However, our understanding of the physiological role of trehalase remains incomplete. Here, we analyze the phenotypes of several Trehalase (Treh) loss-of-function alleles in a comparative manner in Drosophila. The previously reported mutant phenotype of Treh affecting neuroepithelial stem cell maintenance and differentiation in the optic lobe is caused by second-site alleles in addition to Treh. We further report that the survival rate of Treh null mutants is significantly influenced by dietary conditions. Treh mutant larvae are lethal not only on a low-sugar diet but also under low-protein diet conditions. A reduction in adaptation ability under poor food conditions in Treh mutants is mainly caused by the overaccumulation of trehalose rather than the loss of Treh, because the additional loss of Tps1 mitigates the lethal effect of Treh mutants. These results demonstrate that proper trehalose metabolism plays a critical role in adaptation under various environmental conditions.
(MeSH Terms)

Contact
Takashi Nishimura , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Growth Control Signaling
Contributors
Tetsuo Yasugi, Takayuki Yamada, Takashi Nishimura

OMERO Dataset
OMERO Project
Source