Detail of Fig1B_Wnt5a+5b_mutant2



Project
Title
Immunofluorescence images of the tight junction protein Zo-1(red) and the basal body protein Odf2(green) in mouse Wnt5a and Wnt5b mutant embryo
Description
NA
Release, Updated
2018-11-14
License
CC BY-NC-SA
Kind
Image data based on Experiment
File Formats
Data size
1.1 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Gene symbols
Wnt5a, Wnt5b
Protein names
Zo-1, Odf2

Datatype
cell tissue
Molecular Function (MF)
Biological Process (BP)
establishment of cell polarity ( GO:0030010 )
Cellular Component (CC)
ciliary basal body ( GO:0036064 )
Biological Imaging Method
XYZ Scale
XY: 0.105 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
FluorescenceCorrelationSpectroscopy
Contrast method
Fluorescence
Microscope model
Olympus FV-1000
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Minegishi et al. (2017) Dev Cell, 40(5): 439-452.
Related paper(s)

Katsura Minegishi, Masakazu Hashimoto, Rieko Ajima, Katsuyoshi Takaoka, Kyosuke Shinohara, Yayoi Ikawa, Hiromi Nishimura, Andrew P McMahon, Karl Willert, Yasushi Okada, Hiroshi Sasaki, Dongbo Shi, Toshihiko Fujimori, Toshihisa Ohtsuka, Yasunobu Igarashi, Terry P Yamaguchi, Akihiko Shimono, Hidetaka Shiratori, Hiroshi Hamada (2017) A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking., Developmental cell, Volume 40, Number 5, pp. 439-452.e4

Published in 2017 Mar 13

(Abstract) Polarization of node cells along the anterior-posterior axis of mouse embryos is responsible for left-right symmetry breaking. How node cells become polarized has remained unknown, however. Wnt5a and Wnt5b are expressed posteriorly relative to the node, whereas genes for Sfrp inhibitors of Wnt signaling are expressed anteriorly. Here we show that polarization of node cells is impaired in Wnt5a(-/-)Wnt5b(-/-) and Sfrp mutant embryos, and also in the presence of a uniform distribution of Wnt5a or Sfrp1, suggesting that Wnt5 and Sfrp proteins act as instructive signals in this process. The absence of planar cell polarity (PCP) core proteins Prickle1 and Prickle2 in individual cells or local forced expression of Wnt5a perturbed polarization of neighboring wild-type cells. Our results suggest that opposing gradients of Wnt5a and Wnt5b and of their Sfrp inhibitors, together with intercellular signaling via PCP proteins, polarize node cells along the anterior-posterior axis for breaking of left-right symmetry.
(MeSH Terms)

Contact
Hiroshi Hamada , Osaka University , Graduate School of Frontier Biosciences , Developmental Genetics Group
Contributors
Katsura Minegishi, Masakazu Hashimoto, Rieko Ajima, Katsuyoshi Takaoka, Kyosuke Shinohara, Yayoi Ikawa, Hiromi Nishimura, Andrew P. McMahon, Karl Willert, Yasushi Okada, Hiroshi Sasaki, Dongbo Shi, Toshihiko Fujimori, Toshihisa Ohtsuka, Yasunobu Igarashi, Terry P. Yamaguchi, Akihiko Shimono, Hidetaka Shiratori, Hiroshi Hamada

OMERO Dataset
OMERO Project
Source