Detail of Fig1F_E17.5WT_Tuj1+Pax6



Project
Title
Immunohistochemistry staining images of Tuj1 and Pax6 expression in coronal sections of the WT telencephalon at E17.5.
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
2.7 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
NIH3T3
Cell Line
-
Protein names
Tuj1, Pax6

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
neurogenesis ( GO:0022008 ) gliogenesis ( GO:0042063 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
Immunocytochemistry
Microscope type
FluorescenceMicroscope
Acquisition mode
BrightField
Contrast method
Fluorescence
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Bansod et al. (2017) Development, 144(17): 3156-3167.
Related paper(s)

Shama Bansod, Ryoichiro Kageyama, Toshiyuki Ohtsuka (2017) Hes5 regulates the transition timing of neurogenesis and gliogenesis in mammalian neocortical development., Development (Cambridge, England), Volume 144, Number 17, pp. 3156-3167

Published in 2017 Sep 1

(Abstract) During mammalian neocortical development, neural stem/progenitor cells (NSCs) sequentially give rise to deep layer neurons and superficial layer neurons through mid- to late-embryonic stages, shifting to gliogenic phase at perinatal stages. Previously, we found that the Hes genes inhibit neuronal differentiation and maintain NSCs. Here, we generated transgenic mice that overexpress Hes5 in NSCs of the central nervous system, and found that the transition timing from deep to superficial layer neurogenesis was shifted earlier, while gliogenesis precociously occurred in the developing neocortex of Hes5-overexpressing mice. By contrast, the transition from deep to superficial layer neurogenesis and the onset of gliogenesis were delayed in Hes5 knockout (KO) mice. We found that the Hmga genes (Hmga1/2) were downregulated in the neocortical regions of Hes5-overexpressing brain, whereas they were upregulated in the Hes5 KO brain. Furthermore, we found that Hes5 expression led to suppression of Hmga1/2 promoter activity. These results suggest that Hes5 regulates the transition timing between phases for specification of neocortical neurons and between neurogenesis and gliogenesis, accompanied by alteration in the expression levels of Hgma genes, in mammalian neocortical development.
(MeSH Terms)

Contact
Toshiyuki Ohtsuka , Kyoto University , Institute for Frontier Life and Medical Sciences
Contributors
Shama Bansod, Ryoichiro Kageyama, Toshiyuki Ohtsuka

OMERO Dataset
OMERO Project
Source