Detail of fig3D_p21_tTA025_3

Labeling image of mouse neurons with optimization of tetbow plasmid concentrations for in utero electroporation
Release, Updated
Image data based on Experiment

M. musculus ( NCBITaxon:10090 )
Cell Line

neuronal morphology
Molecular Function (MF)
Biological Process (BP)
olfactory behavior ( GO:0042048 ) cell morphogenesis involved in neuron differentiation ( GO:0048667 )
Cellular Component (CC)
dendrite ( GO:00030425 )
Biological Imaging Method
Image Acquisition
ConfocalMicroscope ( TCS SP8X ) , Other , FluorescenceCorrelationSpectroscopy , Fluorescence , Leica HyD , Leica HyD
XYZ Scale
XY: 0.0901435 micrometer/pixel, Z: 0.3345151 micrometer/slice
T scale

Summary of Methods
See details in Sakaguchi et al. (2018) Elife, 7: e40350.
Related paper(s)

Richi Sakaguchi, Marcus N Leiwe, Takeshi Imai (2018) Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags., eLife, Volume 7

Published in 2018 Nov 20 (Electronic publication in Nov. 20, 2018, midnight )

(Abstract) The stochastic multicolor labeling method 'Brainbow' is a powerful strategy to label multiple neurons differentially with fluorescent proteins; however, the fluorescence levels provided by the original attempts to use this strategy were inadequate. In the present study, we developed a stochastic multicolor labeling method with enhanced expression levels that uses a tetracycline-operator system (Tetbow). We optimized Tetbow for either plasmid or virus vector-mediated multicolor labeling. When combined with tissue clearing, Tetbow was powerful enough to visualize the three-dimensional architecture of individual neurons. Using Tetbow, we were able to visualize the axonal projection patterns of individual mitral/tufted cells along several millimeters in the mouse olfactory system. We also developed a Tetbow system with chemical tags, in which genetically encoded chemical tags were labeled with synthetic fluorophores. This was useful in expanding the repertoire of the fluorescence labels and the applications of the Tetbow system. Together, these new tools facilitate light-microscopy-based neuronal tracing at both a large scale and a high resolution.
(MeSH Terms)

Takeshi Imai , Kyushu University , Graduate School of Medical Sciences , Department of Developmental Neurophysiology
Richi Sakaguchi, Marcus N. Leiwe, Takeshi Imai

OMERO Dataset
OMERO Project