Detail of fig6_centrinGFP_Odf2KO



Project
Title
Image of BBs in the Odf2 mutant (Odf2ΔEx6,7ΔEx6,7) GFP-centrin2-transgenic MTECs.
Description
NA
Release, Updated
2018-11-14,
2020-02-03
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
2.0 MB

Organism
M. musculus ( NCBITaxon:10090 )
Strain(s)
-
Cell Line
-
Gene symbols
Odf2
Protein names
centrin2
Protein tags
GFP

Datatype
epithelial cells dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
cytoskeleton ( GO:0005856 )
Biological Imaging Method
XYZ Scale
XY: 0.07 micrometer/pixel, Z: 0.07 micrometer/slice
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
SpinningDiskConfocal
Contrast method
Fluorescence
Microscope model
Olympus SD-OSR
Detector model
ORCA-Flash 4.0 v2; Hamamatsu Photonics
Objective model
Olympus UPlanSApo 60x Sil, ,NA 1.3; 1.6x conversion
Filter set

Summary of Methods
See details in Herawati et al. (2016) J Cell Biol, 214(5): 571-586.
Related paper(s)

Elisa Herawati, Daisuke Taniguchi, Hatsuho Kanoh, Kazuhiro Tateishi, Shuji Ishihara, Sachiko Tsukita (2016) Multiciliated cell basal bodies align in stereotypical patterns coordinated by the apical cytoskeleton., The Journal of cell biology, Volume 214, Number 5, pp. 571-86

Published in 2016 Aug 29

(Abstract) Multiciliated cells (MCCs) promote fluid flow through coordinated ciliary beating, which requires properly organized basal bodies (BBs). Airway MCCs have large numbers of BBs, which are uniformly oriented and, as we show here, align linearly. The mechanism for BB alignment is unexplored. To study this mechanism, we developed a long-term and high-resolution live-imaging system and used it to observe green fluorescent protein-centrin2-labeled BBs in cultured mouse tracheal MCCs. During MCC differentiation, the BB array adopted four stereotypical patterns, from a clustering "floret" pattern to the linear "alignment." This alignment process was correlated with BB orientations, revealed by double immunostaining for BBs and their asymmetrically associated basal feet (BF). The BB alignment was disrupted by disturbing apical microtubules with nocodazole and by a BF-depleting Odf2 mutation. We constructed a theoretical model, which indicated that the apical cytoskeleton, acting like a viscoelastic fluid, provides a self-organizing mechanism in tracheal MCCs to align BBs linearly for mucociliary transport.
(MeSH Terms)

Contact
Sachiko Tsukita, Shuji Ishihara , Osaka University , Graduate School of Frontier Biosciences and Medicine , Laboratory of Biological Science
Contributors
Elisa Herawati, Daisuke Taniguchi, Hatsuho Kanoh, Kazuhiro Tateishi, Shuji Ishihara, Sachiko Tsukita

OMERO Dataset
OMERO Project
Source