Detail of Fig5BC_raw
Project
Title
Stimulated Raman scattering images of TorTED-Drosophila larval fat body.
Description
Stimulated Raman scattering images of deuterated methionine (dMet) uptake in TorTED-Drosophila larval fat body. In TorTED-Drosophila, a dominant negative form of mTOR that interferes and inhibits mTOR signaling in GFP marked cells.The Raman peak of dMet is 2130cm^{-1}. The images of GFP fluorescence in the same area is also reposited.
Release, Updated
2025-04-10
License
CC BY
Kind
Image data
File Formats
.tif
Data size
3.8 MB
Organism
Drosophila
(
NCBI:txid7215
)
Strain(s)
hsFLP, UAS-mCD8-GFP; act5C-FRTstopFRT-GAL4, UAS-GFP, UAS-TorTED
Cell Line
-
Datatype
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Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
X scale
80 micrometer
Y scale
80 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
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Objective model
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Filter set
-
Summary of Methods
Spratt SJ, Mizuguchi T, Akaboshi H, Kosakamoto H, Okada R, Obata F, Ozeki Y. Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering. Front Chem. 2023 Mar 29;11:1141920.
Related paper(s)
Spencer J Spratt, Takaha Mizuguchi, Hikaru Akaboshi, Hina Kosakamoto, Rina Okada, Fumiaki Obata, Yasuyuki Ozeki (2023) Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering., Frontiers in chemistry, Volume 11, pp. 1141920
Published in 2023 (Electronic publication in March 29, 2023, midnight )
- doi: 10.3389/fchem.2023.1141920
-
pmid: 37065821
(Abstract) Introduction: Visualizing small individual biomolecules at subcellular resolution in live cells and tissues can provide valuable insights into metabolic activity in heterogeneous cells, but is challenging. Methods: Here, we used stimulated Raman scattering (SRS) microscopy to image deuterated methionine (d-Met) incorporated into Drosophila tissues in vivo. Results: Our results demonstrate that SRS can detect a range of previously uncharacterized cell-to-cell differences in d-Met distribution within a tissue at the subcellular level. Discussion: These results demonstrate the potential of SRS microscopy for metabolic imaging of less abundant but important amino acids such as methionine in tissue.
Contact
Yasuyuki Ozeki
, The University of Tokyo
, Department of Electrical Engineering and Information Systems
, Department of Electrical Engineering and Information Systems
Contributors
Spencer J Spratt, Takaha Mizuguchi, Hikaru Akaboshi, Hina Kosakamoto
OMERO Project
Source