Detail of FigS1B-2



Project
Title
Co-staining of GFP and neuroendocrine (NE) cell markers Ascl1 and CGRP in RET-EGFP lungs at E18.5 mouse
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
262.1 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Gene symbols
Ascl1
Protein names
RET
Protein tags
EGFP

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.461 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Carl Zeiss LSM710
Detector model
PMT
Objective model
Carl Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC M27
Filter set
-

Summary of Methods
See details in Noguchi et al. (2015) Cell Rep, 13(12): 2679–2686.
Related paper(s)

Masafumi Noguchi, Kenta Sumiyama, Mitsuru Morimoto (2015) Directed Migration of Pulmonary Neuroendocrine Cells toward Airway Branches Organizes the Stereotypic Location of Neuroepithelial Bodies., Cell reports, Volume 13, Number 12, pp. 2679-86

Published in 2015 Dec 29 (Electronic publication in Dec. 17, 2015, midnight )

(Abstract) The airway epithelium consists of diverse cell types, including neuroendocrine (NE) cells. These cells are thought to function as chemoreceptors and as a component of the stem cell niche as well as the cells of origin in small-cell lung cancer. NE cells often localize at bifurcation points of airway tubes, forming small clusters called neuroepithelial bodies (NEBs). To investigate NEB development, we established methods for 3D mapping and ex vivo 4D imaging of developing lungs. We found that NEBs localize at stereotypic positions in the bifurcation area irrespective of variations in size. Notch-Hes1 signaling contributes to the differentiation of solitary NE cells, regulating their number but not localization. Live imaging revealed that individual NE cells migrate distally to and cluster at bifurcation points, driving NEB formation. We propose that NEB development is a multistep process involving differentiation of individual NE cells and their directional migration to organize NEBs.
(MeSH Terms)

Contact
Masafumi Noguchi, Mitsuru Morimoto , RIKEN , Center for Developmental Biology , Laboratory for Lung Development
Contributors
Masafumi Noguchi, Kenta Sumiyama, Mitsuru Morimoto

OMERO Dataset
OMERO Project
Source