Detail of Video2_pallium
Project
SSBD:Repository
Title
Z-stack images of medaka brain that visualize clonally related units in the pallium
Description
Z-series images of medaka brain obtained by light sheet microscopy that visualize clonally related units in the pallium. This transgenic line express fluorescent protein under HuC promoter, which drives expression in neural progenitor cells. As the reporter GFP was expressed in neural progenitor cells at the neurula stage (stage 16–17) by Cre-loxp recombination system, we can trace the lineage in the adult fish. GFP-positive cells (channel2) make a cluster and send a projection to the same site. Red signals indicate DsRed (channel1) and blue signals indicate DAPI staining (channel3). Most of the GFP-positive cells in the pallium are not mixed with DsRed-positive cells and did not locate across multiple regions. In this brain, the clonally related units are detected in Dld and Dd2 region of the pallium.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
.tif
Data size
331.1 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neurogenesis
(
GO:0022008
)
,
neuron migration
(
GO:0001764
)
Cellular Component (CC)
Biological Imaging Method
light-sheet microscopy
X scale
-
Y scale
-
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Isoe Y, Nakamura R, Nonaka S, Kamei Y, Okuyama T, Yamamoto N, Takeuchi H, Takeda H. Epigenetically distinct synaptic architecture in clonal compartments in the teleostean dorsal pallium. Elife. 2023 Jul 25;12:e85093. doi: 10.7554/eLife.85093.
Related paper(s)
Contact
Yasuko Isoe
, Harvard University
, Department of Molecular and Cellular Biology
, Department of Molecular and Cellular Biology
Contributors
Ryohei Nakamura
OMERO Project