Detail of Video2_pallium
Project
Title
Z-stack images of medaka brain that visualize clonally related units in the pallium
Description
Z-series images of medaka brain obtained by light sheet microscopy that visualize clonally related units in the pallium. This transgenic line express fluorescent protein under HuC promoter, which drives expression in neural progenitor cells. As the reporter GFP was expressed in neural progenitor cells at the neurula stage (stage 16–17) by Cre-loxp recombination system, we can trace the lineage in the adult fish. GFP-positive cells (channel2) make a cluster and send a projection to the same site. Red signals indicate DsRed (channel1) and blue signals indicate DAPI staining (channel3). Most of the GFP-positive cells in the pallium are not mixed with DsRed-positive cells and did not locate across multiple regions. In this brain, the clonally related units are detected in Dld and Dd2 region of the pallium.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
.tif
Data size
331.1 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neurogenesis
(
GO:0022008
)
neuron migration
(
GO:0001764
)
Cellular Component (CC)
Biological Imaging Method
light-sheet microscopy
X scale
-
Y scale
-
Z scale
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T scale
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Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
Isoe Y, Nakamura R, Nonaka S, Kamei Y, Okuyama T, Yamamoto N, Takeuchi H, Takeda H. Epigenetically distinct synaptic architecture in clonal compartments in the teleostean dorsal pallium. Elife. 2023 Jul 25;12:e85093. doi: 10.7554/eLife.85093.
Related paper(s)
Yasuko Isoe, Ryohei Nakamura, Shigenori Nonaka, Yasuhiro Kamei, Teruhiro Okuyama, Naoyuki Yamamoto, Hideaki Takeuchi, Hiroyuki Takeda (2023) Epigenetically distinct synaptic architecture in clonal compartments in the teleostean dorsal pallium., eLife, Volume 12
Published in 2023 Jul 25 (Electronic publication in July 25, 2023, midnight )
- doi: 10.7554/eLife.85093
-
pmid: 37489039
(Abstract) The dorsal telencephalon (i.e. the pallium) exhibits high anatomical diversity across vertebrate classes. The non-mammalian dorsal pallium accommodates various compartmentalized structures among species. The developmental, functional, and evolutional diversity of the dorsal pallium remain unillustrated. Here, we analyzed the structure and epigenetic landscapes of cell lineages in the telencephalon of medaka fish (Oryzias latipes) that possesses a clearly delineated dorsal pallium (Dd2). We found that pallial anatomical regions, including Dd2, are formed by mutually exclusive clonal units, and that each pallium compartment exhibits a distinct epigenetic landscape. In particular, Dd2 possesses a unique open chromatin pattern that preferentially targets synaptic genes. Indeed, Dd2 shows a high density of synapses. Finally, we identified several transcription factors as candidate regulators. Taken together, we suggest that cell lineages are the basic components for the functional regionalization in the pallial anatomical compartments and that their changes have been the driving force for evolutionary diversity.
Contact
Yasuko Isoe
, Harvard University
, Department of Molecular and Cellular Biology
, Department of Molecular and Cellular Biology
Contributors
Ryohei Nakamura
OMERO Project
Source