Detail of Fig5ab_TensionWater
Project
Title
Time-lapse images of tension sensor in VSMCs isolated from the aorta before and after stimulation with distilled water
Description
Time-lapse images of actinin tension sensor in VSMCs (Vascular smooth muscle cells) isolated from the aorta before and after stimulation with distilled water. Decrease in energy transfer from EGFP to mCherry reflects the increase in cell tension. Water was added between 20-20.5 min.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
.czi
Data size
1.9 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
response to osmotic stress
Cellular Component (CC)
cytoskeleton
Biological Imaging Method
FRET
(
Fbbi:00000367
)
confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )
confocal microscopy ( Fbbi:00000251 )
time lapse microscopy ( Fbbi:00000249 )
X scale
0.264 micrometer/pixel
Y scale
0.264 micrometer/pixel
Z scale
0.5 micrometer/slice
T scale
0.5 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Wang J, Maeda E, Tsujimura Y, Abe T, Kiyonari H, Kitaguchi T, Yokota H, Matsumoto T. In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor. Sci Rep. 2023 Dec 20;13(1):22729.
Related paper(s)
Junfeng Wang, Eijiro Maeda, Yuki Tsujimura, Takaya Abe, Hiroshi Kiyonari, Tetsuya Kitaguchi, Hideo Yokota, Takeo Matsumoto (2023) In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor., Scientific reports, Volume 13, Number 1, pp. 22729
Published in 2023 Dec 20 (Electronic publication in Dec. 20, 2023, midnight )
- doi: 10.1038/s41598-023-50142-z
-
pmid: 38123655
(Abstract) FRET-based sensors are utilized for real-time measurements of cellular tension. However, transfection of the sensor gene shows low efficacy and is only effective for a short period. Reporter mice expressing such sensors have been developed, but sensor fluorescence has not been measured successfully using conventional confocal microscopy. Therefore, methods for spatiotemporal measurement of cellular tension in vivo or ex vivo are still limited. We established a reporter mouse line expressing FRET-based actinin tension sensors consisting of EGFP as the donor and mCherry as the acceptor and whose FRET ratio change is observable with confocal microscopy. Tension-induced changes in FRET signals were monitored in the aorta and tail tendon fascicles, as well as aortic smooth muscle cells isolated from these mice. The pattern of FRET changes was distinctive, depending on tissue type. Indeed, aortic smooth muscle cells exhibit different sensitivity to macroscopic tensile strain in situ and in an isolated state. This mouse strain will enable novel types of biomechanical investigations of cell functions in important physiological events.(MeSH Terms)
Contact
Takeo Matsumoto
, Nagoya University
, Biomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering
, Biomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering
Contributors
Junfeng Wang, Yuki Tsujimura, Takaya Abe, Hiroshi Kiyonari, Tetsuya Kitaguchi
OMERO Project
Source