Detail of FigS3AB_MTcompact


Project
SSBD:Repository
Title
Time-series images of GDP-MT and KIF5C before and after washout of KIF5C
Description
Time-series images of GDP-MT (GDP-microtubules) and monomeric KIF5C (K351) before and after washout of KIF5C, which were obtained by fluorescent speckle microscopy.
Release, Updated
2024-12-14
License
CC BY
Kind
Image data
File Formats
tif
Data size
42.6 MB

Organism
-
Strain(s)
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Cell Line
-

Datatype
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Molecular Function (MF)
Biological Process (BP)
microtubule depolymerization
Cellular Component (CC)
Biological Imaging Method
evanescent wave microscopy ( Fbbi:00000617 )
time lapse microscopy ( Fbbi:00000249 )
X scale
60 nanometer/pixel
Y scale
60 nanometer/pixel
Z scale
-
T scale
2 seconds per time interval, 5 seconds per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N. Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport. J Cell Biol. 2018 Dec 3;217(12):4164-4183.
Related paper(s)

Contact
Yasushi Okada, Nobutaka Hirokawa , RIKEN BDR, The University of Tokyo , Laboratory for Cell Polarity Regulation, Department of Cell Biology and Anatomy, Graduate School of Medicine , Laboratory for Cell Polarity Regulation, Department of Cell Biology and Anatomy, Graduate School of Medicine
Contributors
Tomohiro Shima, Sotaro Uemura, Yasushi Okada

OMERO Dataset
OMERO Project
Source