Detail of extended_glucose-fed_wild-type



Project
SSBD:Repository
Title
Time-series images of behavioral activity of wild-type C. elegans cultured in perfusing M9 buffer with 1 g/L glucose.
Description
Time-series images of behavioral activity of wild-type C. elegans cultured in perfusing M9 buffer with 1 g/L glucose.
Release, Updated
2023-05-11
License
CC BY
Kind
Image data
File Formats
.mp4
Data size
9.9 GB

Organism
Caenorhabditis elegans ( NCBITaxon:6239 )
Strain(s)
Bristol N2
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
behavior ( GO:0007610 )
Cellular Component (CC)
Biological Imaging Method
bright-field microscopy ( Fbbi:00000243 )
X scale
-
Y scale
-
Z scale
-
T scale
20 frames per second

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
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Objective model
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Filter set
-

Summary of Methods
See details in Arata Y, et. al. (2022) Sci Rep. 2022 Jun 21;12(1):10481.
Related paper(s)

Yukinobu Arata, Itsuki Shiga, Yusaku Ikeda, Peter Jurica, Hiroshi Kimura, Ken Kiyono, Yasushi Sako (2022) Insulin signaling shapes fractal scaling of C. elegans behavior., Scientific reports, Volume 12, Number 1, pp. 10481

Published in 2022 Jun 21 (Electronic publication in June 21, 2022, midnight )

(Abstract) Fractal scaling in animal behavioral activity, where similar temporal patterns appear repeatedly over a series of magnifications among time scales, governs the complex behavior of various animal species and, in humans, can be altered by neurodegenerative diseases and aging. However, the mechanism underlying fractal scaling remains unknown. Here, we cultured C. elegans in a microfluidic device for 3 days and analyzed temporal patterns of C. elegans activity by fractal analyses. The residence-time distribution of C. elegans behaviors shared a common feature with those of human and mice. Specifically, the residence-time power-law distribution of the active state changed to an exponential-like decline at a longer time scale, whereas the inactive state followed a power-law distribution. An exponential-like decline appeared with nutrient supply in wild-type animals, whereas this decline disappeared in insulin-signaling-defective daf-2 and daf-16 mutants. The absolute value of the power-law exponent of the inactive state distribution increased with nutrient supply in wild-type animals, whereas the value decreased in daf-2 and daf-16 mutants. We conclude that insulin signaling differentially affects mechanisms that determine the residence time in active and inactive states in C. elegans behavior. In humans, diabetes mellitus, which is caused by defects in insulin signaling, is associated with mood disorders that affect daily behavioral activities. We hypothesize that comorbid behavioral defects in patients with diabetes may be attributed to altered fractal scaling of human behavior.
(MeSH Terms)

Contact
Yukinobu Arata , RIKEN , Cluster for Pioneering Research (CPR) , Cellular Informatics Laboratory
Contributors

OMERO Dataset
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OMERO Project
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Source