Detail of Fig5_B-gTEMP_heat_transfer



Project
Title
Time-series image of temperature inside HeLa cell expressing B-gTEMP during heat diffusion
Description
Time-series image of temperature inside HeLa cell expressing B-gTEMP during heat diffusion
Release, Updated
2023-03-22
License
CC BY
Kind
Image data
File Formats
.tif
Data size
31.3 MB

Organism
Homo sapiens ( NCBI:txid9606 )
Strain(s)
-
Cell Line
HeLa cell ( CLO_0003684 )
Reporter
B-gTEMP

Datatype
-
Molecular Function (MF)
Biological Process (BP)
heat dissipation ( GO:0031653 )
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
X scale
290 nanometer/pixel
Y scale
290 nanometer/pixel
Z scale
-
T scale
155 microseconds per frame

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Lu K, et. al. (2022) Nano Lett, 22(14):5698-5707.
Related paper(s)

Kai Lu, Tetsuichi Wazawa, Joe Sakamoto, Cong Quang Vu, Masahiro Nakano, Yasuhiro Kamei, Takeharu Nagai (2022) Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer., Nano letters, Volume 22, Number 14, pp. 5698-5707

Published in 2022 Jul 27 (Electronic publication in July 6, 2022, midnight )

(Abstract) Despite improved sensitivity of nanothermometers, direct observation of heat transport inside single cells has remained challenging for the lack of high-speed temperature imaging techniques. Here, we identified insufficient temperature resolution under short signal integration time and slow sensor kinetics as two major bottlenecks. To overcome the limitations, we developed B-gTEMP, a nanothermometer based on the tandem fusion of mNeonGreen and tdTomato fluorescent proteins. We visualized the propagation of heat inside intracellular space by tracking the temporal variation of local temperature at a time resolution of 155 mus and a temperature resolution 0.042 degrees C. By comparing the fast in situ temperature dynamics with computer-simulated heat diffusion, we estimated the thermal diffusivity of live HeLa cells. The present thermal diffusivity in cells was about 1/5.3 of that of water and much smaller than the values reported for bulk tissues, which may account for observations of heterogeneous intracellular temperature distributions.
(MeSH Terms)

Contact
Takeharu Nagai , Osaka University , SANKEN , Department of Biomolecular Science and Engineering
Contributors
Kai Lu, Tetsuichi WazawaKai Lu, Tetsuichi Wazawa

OMERO Dataset
OMERO Project
Source