Detail of Fig1a_MI_Young



Project
Title
Time-lapse microscopy images of oocytes expressing 2mEGFP-CENP-C and H2B-mCherry in a young (2 months old) BDF1 mouse
Description
NA
Release, Updated
2017-10-03,
2018-11-15
License
CC BY-NC-SA
Kind
Image data based on Experiment
File Formats
Data size
1.8 GB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Protein names
CENP, H2B
Protein tags
2mEGFP, mCherry

Datatype
kinetochore dynamics
Molecular Function (MF)
Biological Process (BP)
meiosis I ( GO:0007127 )
Cellular Component (CC)
kinetochore ( GO:0000776 ) chromosome ( GO:0005694 )
Biological Imaging Method
XYZ Scale
XY: 0.1186094 micrometer/pixel, Z: 1.5 micrometer/frame
T scale
3 minute for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Zeiss LSM710
Detector model
-
Objective model
Zeiss C-Apochromat 40x/1.2NA W
Filter set
-

Summary of Methods
See details in Sakakibara et al. (2015) Nat Commun. 2013; 4:2212.
Related paper(s)

Yogo Sakakibara, Shu Hashimoto, Yoshiharu Nakaoka, Anna Kouznetsova, Christer Hoog, Tomoya S Kitajima (2015) Bivalent separation into univalents precedes age-related meiosis I errors in oocytes., Nature communications, Volume 6, pp. 7550

Published in 2015 Jul 1 (Electronic publication in July 1, 2015, midnight )

(Abstract) The frequency of chromosome segregation errors during meiosis I (MI) in oocytes increases with age. The two-hit model suggests that errors are caused by the combination of a first hit that creates susceptible crossover configurations and a second hit comprising an age-related reduction in chromosome cohesion. This model predicts an age-related increase in univalents, but direct evidence of this phenomenon as a major cause of segregation errors has been lacking. Here, we provide the first live analysis of single chromosomes undergoing segregation errors during MI in the oocytes of naturally aged mice. Chromosome tracking reveals that 80% of the errors are preceded by bivalent separation into univalents. The set of the univalents is biased towards balanced and unbalanced predivision of sister chromatids during MI. Moreover, we find univalents predisposed to predivision in human oocytes. This study defines premature bivalent separation into univalents as the primary defect responsible for age-related aneuploidy.
(MeSH Terms)

Contact
Tomoya Kitajima , RIKEN , Center for Developmental Biology , Laboratory for Chromosome Segregation
Contributors
Yogo Sakakibara, Tomoya Kitajima

OMERO Dataset
OMERO Project
Source