Detail of wtPAR-2pkc-3
Project
Title
Time-lapse semi-TIRF microscopy images of Fluorescent particles of GFP::PAR-2 in a C. elegans pkc-3(RNAi) embryo
Description
NA
Release, Updated
2017-10-03,
2020-02-03
2020-02-03
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
504.2 MB
Gene symbols
pkc-3
Protein names
PAR-2
Protein tags
GFP
Datatype
single molecule dynamics
Molecular Function (MF)
Biological Process (BP)
cellular protein localization
(
GO:0034613
)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.0667 micrometer/pixel, Z: NA
T scale
0.05 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
TIRF
Contrast method
Fluorescence
Microscope model
Nikon TE2000
Detector model
Hamamatsu Photonics ImagEM
Objective model
Nikon Apo TIRF 60X/1.49
Filter set
Summary of Methods
See details in Arata et al. (2016) Cell Reports, 16, 1-13.
Related paper(s)
Yukinobu Arata, Michio Hiroshima, Chan-Gi Pack, Ravikrishna Ramanujam, Fumio Motegi, Kenichi Nakazato, Yuki Shindo, Paul W Wiseman, Hitoshi Sawa, Tetsuya J Kobayashi, Hugo B Brandao, Tatsuo Shibata, Yasushi Sako (2016) Cortical Polarity of the RING Protein PAR-2 Is Maintained by Exchange Rate Kinetics at the Cortical-Cytoplasmic Boundary., Cell reports, Volume 16, Number 8, pp. 2156-2168
Published in 2016 Aug 23 (Electronic publication in Aug. 11, 2016, midnight )
- doi: 10.1016/j.celrep.2016.07.047
-
pmid: 27524610
(Abstract) Cell polarity arises through the spatial segregation of polarity regulators. PAR proteins are polarity regulators that localize asymmetrically to two opposing cortical domains. However, it is unclear how the spatially segregated PAR proteins interact to maintain their mutually exclusive partitioning. Here, single-molecule detection analysis in Caenorhabditis elegans embryos reveals that cortical PAR-2 diffuses only short distances, and, as a result, most PAR-2 molecules associate and dissociate from the cortex without crossing into the opposing domain. Our results show that cortical PAR-2 asymmetry is maintained by the local exchange reactions that occur at the cortical-cytoplasmic boundary. Additionally, we demonstrate that local exchange reactions are sufficient to maintain cortical asymmetry in a parameter-free mathematical model. These findings suggest that anterior and posterior PAR proteins primarily interact through the cytoplasmic pool and not via cortical diffusion.(MeSH Terms)
- Animals
- Caenorhabditis elegans/cytology
- Caenorhabditis elegans/genetics
- Caenorhabditis elegans/metabolism[major]
- Caenorhabditis elegans Proteins/genetics
- Caenorhabditis elegans Proteins/metabolism[major]
- Cell Compartmentation
- Cell Polarity
- Cytoplasm/metabolism[major]
- Cytoplasm/ultrastructure
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism[major]
- Gene Expression Regulation
- Kinetics
- Models, Statistical[major]
- Phosphorylation
- Protein Transport
- Single Molecule Imaging
Contact
Yukinobu Arata, Yasushi Sako
, RIKEN
, Wako
, Cellular Informatics Laboratory
Contributors
Yukinobu Arata, Michio Hiroshima, Chan-Gi Pack, Ravikrishna Ramanujam, Fumio Motegi, Kenichi Nakazato, Yuki Shindo, Paul W. Wiseman, Hitoshi Sawa, Tetsuya J. Kobayashi, Hugo B. Brandão, Tatsuo Shibata, Yasushi Sako
OMERO Dataset
OMERO Project
Source