Summary of 274-Aini-MitochondrialDyn

SSBD:database
SSBD:database URL
Title
Fluorescence images or time-lapse images of mitochondrial distribution dynamics during the development of rice zygotes
Description
-
Relase date
2023-05-29
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
34 ( Image datasets: 34, Quantitative data datasets: 0 )
Size of Datasets
179.4 MB ( Image datasets: 179.4 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Oryza sativa temperate japonica subgroup
Gene symbol(s)
NA
Protein name(s)
NA
Reporter(s)
MitoTracker Red, MitoTrackerRed, PC3

Datatype
-
Molecular Function (MF)
Biological Process (BP)
mitochondrion distribution
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy, fluorescence microscopy
X scale
0.1317882 micrometer/pixel, 0.1054306 micrometer/pixel, 0.2075665 micrometer/pixel
Y scale
0.1317882 micrometer/pixel, 0.1054306 micrometer/pixel, 0.2075665 micrometer/pixel
Z scale
-
T scale
2 seconds of time interval, NA

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Hanifah Aini, Yoshikatsu Sato, Kakishi Uno, Tetsuya Higashiyama, Takashi Okamoto (2022) Dynamics of mitochondrial distribution during development and asymmetric division of rice zygotes., Plant reproduction, Volume 35, Number 1, pp. 47-60

Published in 2022 Mar (Electronic publication in Oct. 11, 2021, midnight )

(Abstract) KEY MESSAGE: Mitochondria change their distribution from nuclear peripheral to uniformly distributed in cytoplasm during zygotic development of rice, and the mitochondria re-distribute around nucleus for even segregation into daughter cells. Mitochondria are highly dynamic organelles that actively move and change their localization along with actin filaments during the cell cycle. Studies of mitochondrial dynamics and distribution in plant cells have mainly been conducted on somatic cells, and our understanding about these aspects during the formation and development of zygotes remains limited. In this study, mitochondrial nucleoids of rice egg cells and zygotes were successfully stained by using N-aryl pyrido cyanine 3 (PC3), and their intracellular localization and distribution were demonstrated. Mitochondria in rice egg cells were small and coccoid in shape and were primarily distributed around the nucleus. Upon gamete fusion, the resulting zygotes showed mitochondrial dispersion and accumulation equivalent to those in rice egg cells until 8 h after fusion (HAF). Around 12 HAF, the mitochondria started to disperse throughout the cytoplasm of the zygotes, and this dispersive distribution pattern continued until the zygotes entered the mitotic phase. At early prophase, the mitochondria redistributed from dispersive to densely accumulated around the nucleus, and during the metaphase and anaphase, the mitochondria were depleted from possible mitotic spindle region. Thereafter, during cell plate formation between daughter nuclei, the mitochondria distributed along the phragmoplast, where the new cell wall was formed. Finally, relatively equivalent amounts of mitochondria were detected in the apical and basal cells which were produced through asymmetric division of the zygotes. Further observation by treating the egg cell with latrunculin B revealed that the accumulation of mitochondria around the nuclear periphery in egg cells and early zygotes depended on the actin meshwork converging toward the egg or zygote nucleus.
(MeSH Terms)

Contact
Takashi Okamoto , Tokyo Metropolitan University , Department of Biological Sciences
Contributors


Dataset List of 274-Aini-MitochondrialDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 10679
Datast ID Figure2A_PC3_1Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10680
Datast ID Figure2A_PC3_4Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10681
Datast ID Figure2A_PC3_8Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10682
Datast ID Figure2A_PC3_12Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10683
Datast ID Figure2A_PC3_16Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10684
Datast ID Figure2A_PC3_18Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10685
Datast ID Figure2A_PC3_19Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10686
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10687
Datast ID Figure2C_PC3_1Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10688
Datast ID Figure2C_PC3_4Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10689
Datast ID Figure2C_PC3_8Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10690
Datast ID Figure2C_PC3_12Haf
Dataset Kind Image data
Dataset Size 30.0 MB
4D view
SSBD:OMERO
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# 10691
Datast ID Figure2C_PC3_19Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10692
Dataset Kind Image data
Dataset Size 30.0 MB
4D view
SSBD:OMERO
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# 10693
Datast ID Figure2C_PC3_20Haf
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10694
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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# 10695
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10696
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10697
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10698
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10699
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10700
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10701
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10702
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10703
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10704
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10705
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10706
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10707
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10708
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10709
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10710
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 10711
Dataset Kind Image data
Dataset Size 15.1 MB
4D view
SSBD:OMERO
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# 10712
Dataset Kind Image data
Dataset Size 15.1 MB
4D view
SSBD:OMERO
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