Summary of 127-Konishi-ERKDyn

SSBD:database
SSBD:database URL
Title
Live-Cell FRET images and BDML quantitative information about extracellular signal-regulated kinase activity dynamics in thymocyte motility
Description
-
Relase date
2021-09-30
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
143 ( Image datasets: 143, Quantitative data datasets: 0 )
Size of Datasets
26.4 GB ( Image datasets: 26.4 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Gene symbol(s)
NA
Protein name(s)
ERK, MHC-II

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
cell motility, activation of MAPERK kinase
Cellular Component (CC)
T cell receptor complex
Biological Imaging Method
time lapse microscopy, fluorescence microscopy
X scale
0.994 micrometer/pixel, 0.454 micrometer/pixel, 0.795 micrometer/pixel, 0.53 micrometer/pixel, 0.497 micrometer/pixel, 0.662 micrometer/pixel, 0.636 micrometer/pixel, 0.22 micrometer/pixel
Y scale
0.994 micrometer/pixel, 0.454 micrometer/pixel, 0.795 micrometer/pixel, 0.53 micrometer/pixel, 0.497 micrometer/pixel, 0.662 micrometer/pixel, 0.636 micrometer/pixel, 0.22 micrometer/pixel
Z scale
2 micrometer/slice, 3 micrometer/slice, 1 micrometer/slice
T scale
33.63 second for each time interval, 31.01 second for each time interval, 33.66 second for each time interval, 33.72 second for each time interval, 30.56 second for each time interval, 35.35 second for each time interval, 75.3 second for each time interval, 35.32 second for each time interval, 33.55 second for each time interval, 33.87 second for each time interval, 30.57 second for each time interval, 35.24 second for each time interval, 15.01 second for each time interval, 33.71 second for each time interval, 33.73 second for each time interval, 53.16 second for each time interval, 33.59 second for each time interval, 40.8 second for each time interval, 33.61 second for each time interval, 30.74 second for each time interval, 30.93 second for each time interval, 33.53 second for each time interval, 31.77 second for each time interval, 33.57 second for each time interval, NA, 37.06 second for each time interval, 33.88 second for each time interval, 31 second for each time interval, 30.53 second for each time interval, 33.47 second for each time interval, 33.83 second for each time interval, 33.96 second for each time interval, 33.58 second for each time interval, 34.8 second for each time interval, 33.7 second for each time interval, 33.64 second for each time interval, 1 second for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Yoshinobu Konishi, Kenta Terai, Yasuhide Furuta, Hiroshi Kiyonari, Takaya Abe, Yoshihiro Ueda, Tatsuo Kinashi, Yoko Hamazaki, Akifumi Takaori-Kondo, Michiyuki Matsuda (2018) Live-Cell FRET Imaging Reveals a Role of Extracellular Signal-Regulated Kinase Activity Dynamics in Thymocyte Motility., iScience, Volume 10, pp. 98-113

Published in 2018 Dec 21 (Electronic publication in Nov. 20, 2018, midnight )

(Abstract) Extracellular signal-regulated kinase (ERK) plays critical roles in T cell development in the thymus. Nevertheless, the dynamics of ERK activity and the role of ERK in regulating thymocyte motility remain largely unknown due to technical limitations. To visualize ERK activity in thymocytes, we here developed knockin reporter mice expressing a Forster/fluorescence resonance energy transfer (FRET)-based biosensor for ERK from the ROSA26 locus. Live imaging of thymocytes isolated from the reporter mice revealed that ERK regulates thymocyte motility in a subtype-specific manner. Negative correlation between ERK activity and motility was observed in CD4/CD8 double-positive thymocytes and CD8 single-positive thymocytes, but not in CD4 single-positive thymocytes. Interestingly, however, the temporal deviations of ERK activity from the average correlate with the motility of CD4 single-positive thymocytes. Thus, live-cell FRET imaging will open a window to understanding the dynamic nature and the diverse functions of ERK signaling in T cell biology.

Contact
Kenta Terai , Kyoto University , Graduate School of Biostudies , Laboratory of Bioimaging and Cell Signaling
Contributors


Dataset List of 127-Konishi-ERKDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 5572
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5573
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5574
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5575
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5576
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5577
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5578
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5579
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5580
Datast ID Figure1G_ERK_MEKi
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5581
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5582
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5583
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5584
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5585
Dataset Kind Image data
Dataset Size 11.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5586
Datast ID Figure3D_cortex
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5587
Datast ID Figure3D_medulla
Dataset Kind Image data
Dataset Size 527.9 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5588
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5589
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5590
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5591
Dataset Kind Image data
Dataset Size 28.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5592
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5593
Dataset Kind Image data
Dataset Size 527.9 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5594
Dataset Kind Image data
Dataset Size 328.4 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5595
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5596
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5597
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5598
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5599
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5600
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5601
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5602
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5603
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5604
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5605
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5606
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5607
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5608
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5609
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5610
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5611
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5612
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5613
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5614
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5615
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5616
Dataset Kind Image data
Dataset Size 397.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5617
Dataset Kind Image data
Dataset Size 397.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5618
Dataset Kind Image data
Dataset Size 363.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5619
Dataset Kind Image data
Dataset Size 92.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5620
Dataset Kind Image data
Dataset Size 353.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5621
Dataset Kind Image data
Dataset Size 353.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5622
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5623
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5624
Dataset Kind Image data
Dataset Size 440.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5625
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5626
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5627
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5628
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5629
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5630
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5631
Dataset Kind Image data
Dataset Size 28.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5632
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5633
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5634
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5635
Datast ID Figure4D_ERK_0422
Dataset Kind Image data
Dataset Size 32.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5636
Datast ID Figure4D_ERK_0513
Dataset Kind Image data
Dataset Size 28.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5637
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5638
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5639
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5640
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5641
Datast ID Figure5C_MEKi_DP1
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5642
Datast ID Figure5C_MEKi_DP2
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5643
Datast ID Figure5C_MEKi_DP3
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5644
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5645
Dataset Kind Image data
Dataset Size 397.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5646
Dataset Kind Image data
Dataset Size 397.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5647
Dataset Kind Image data
Dataset Size 363.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5648
Dataset Kind Image data
Dataset Size 92.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5649
Dataset Kind Image data
Dataset Size 353.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5650
Dataset Kind Image data
Dataset Size 353.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5651
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5652
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5653
Dataset Kind Image data
Dataset Size 440.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5654
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5655
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5656
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5657
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5658
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5659
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5660
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5661
Dataset Kind Image data
Dataset Size 320.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5662
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5663
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5664
Datast ID Figure5DF_MEKi_DP1
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5665
Datast ID Figure5DF_MEKi_DP2
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5666
Datast ID Figure5DF_MEKi_DP3
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5667
Datast ID Figure6AC_CD4_0522
Dataset Kind Image data
Dataset Size 58.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5668
Dataset Kind Image data
Dataset Size 397.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5669
Dataset Kind Image data
Dataset Size 397.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5670
Dataset Kind Image data
Dataset Size 363.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5671
Datast ID Figure6AC_CD8_0522
Dataset Kind Image data
Dataset Size 92.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5672
Dataset Kind Image data
Dataset Size 353.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5673
Dataset Kind Image data
Dataset Size 353.4 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5674
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5675
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5676
Dataset Kind Image data
Dataset Size 440.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5677
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5678
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5679
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5680
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5681
Dataset Kind Image data
Dataset Size 481.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5682
Dataset Kind Image data
Dataset Size 481.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5683
Dataset Kind Image data
Dataset Size 481.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5684
Dataset Kind Image data
Dataset Size 481.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5685
Dataset Kind Image data
Dataset Size 481.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5686
Dataset Kind Image data
Dataset Size 481.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5687
Dataset Kind Image data
Dataset Size 161.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5688
Dataset Kind Image data
Dataset Size 329.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5689
Dataset Kind Image data
Dataset Size 481.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5690
Dataset Kind Image data
Dataset Size 481.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5691
Dataset Kind Image data
Dataset Size 329.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5692
Dataset Kind Image data
Dataset Size 329.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5693
Dataset Kind Image data
Dataset Size 249.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5694
Dataset Kind Image data
Dataset Size 329.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5695
Dataset Kind Image data
Dataset Size 329.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5696
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5697
Dataset Kind Image data
Dataset Size 11.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5698
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5699
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5700
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5701
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5702
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5703
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5704
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5705
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5706
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5707
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5708
Datast ID Video1_afterCD3
Dataset Kind Image data
Dataset Size 13.2 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5709
Dataset Kind Image data
Dataset Size 164.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5710
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5711
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5712
Dataset Kind Image data
Dataset Size 321.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5713
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 5714
Dataset Kind Image data
Dataset Size 343.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data