Summary of 106-Jin-BreastTumorFlu

SSBD:database
SSBD:database URL
Title
Bright field and NIR fluorescence images of breast tumor mouse injected with ICG-Ab contatining S4-6 micelles or without S4-6 micelles for several time course
Description
-
Relase date
2018-11-14
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
8 ( Image datasets: 8, Quantitative data datasets: 0 )
Size of Datasets
4.0 MB ( Image datasets: 4.0 MB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Strain(s)
KPL-4
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
breast tumor fluorescence
Molecular Function (MF)
Biological Process (BP)
naphthalenesulfonate metabolic process
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
FluorescenceMicroscope
Acquisition mode
FluorescenceCorrelationSpectroscopy
Contrast method
Fluorescence
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Jin, Takashi, Tsuboi, Setsuko, Komatsuzaki, Akihito, Imamura, Yukio, Muranaka, Yoshinori, Sakata, Takao, Yasuda, Hidehiro (2016), Enhancement of aqueous stability and fluorescence brightness of indocyanine green using small calix[4]arene micelles for near-infrared fluorescence imaging, Med. Chem. Commun., Volume 7, Number 4, 623-631

Published in 2016

(Abstract) Indocyanine green (ICG) is the only near-infrared (NIR) fluorescent dye which is approved for medical applications. However, ICG has several drawbacks such as aqueous instability, photodegradation, and low fluorescence quantum yield (2.5% in water), which lead to the limitation on the use of ICG for in vitro and in vivo NIR fluorescence imaging. Free ICG rapidly aggregates in physiological buffer solutions, and its fluorescence diminishes within several days. The objective of this work is to provide an easy method for the enhancement of the stability and fluorescence brightness of ICG in aqueous solutions for NIR fluorescence imaging. Herein, we report that the incorporation of ICG into small calix[4]arene (S4-6) micelles (<5 nm in diameter) significantly improves the aqueous stability and fluorescence brightness of ICG. The fluorescence quantum yields of ICG-calix[4]arene micelles are increased up to ∼6% in aqueous solutions. Using the ICG-calix[4]arene micelles, we achieved non-invasive NIR fluorescence imaging of the liver and lymph system in mice. Furthermore, we achieved NIR fluorescence imaging of nude mice bearing human breast tumors using an ICG conjugated antibody which is incorporated into the calix[4]arene micelles. Preparation of the calix[4]arene micelles including ICG is very easy and the micelle system does not show significant cytotoxicity. The ICG-calix[4]arene micelle system acts as a highly stable and bright probe for in vitro and in vivo NIR fluorescence imaging.

Contact
Takashi Jin , RIKEN , Quantitative Biology Center , Laboratory for Nano-Bio Probes
Contributors
Takashi Jin, Setsuko Tsuboi, Akihito Komatsuzaki, Yukio Imamura, Yoshinori Muranaka, Takao Sakata, Hidehiro Yasuda


Dataset List of 106-Jin-BreastTumorFlu

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 4405
Datast ID fig10a-1
Dataset Kind Image data
Dataset Size 196.4 KB
4D view
SSBD:OMERO
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# 4406
Datast ID fig10a-2
Dataset Kind Image data
Dataset Size 755.7 KB
4D view
SSBD:OMERO
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# 4407
Datast ID fig10b-1
Dataset Kind Image data
Dataset Size 554.0 KB
4D view
SSBD:OMERO
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# 4408
Datast ID fig10b-2
Dataset Kind Image data
Dataset Size 541.1 KB
4D view
SSBD:OMERO
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# 4409
Datast ID fig10b-3
Dataset Kind Image data
Dataset Size 537.6 KB
4D view
SSBD:OMERO
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# 4410
Datast ID fig10b-4
Dataset Kind Image data
Dataset Size 509.7 KB
4D view
SSBD:OMERO
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# 4411
Datast ID fig10b-5
Dataset Kind Image data
Dataset Size 520.6 KB
4D view
SSBD:OMERO
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# 4412
Datast ID fig10b-6
Dataset Kind Image data
Dataset Size 506.8 KB
4D view
SSBD:OMERO
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