386-Takahashi-EarIL31RA
Author reviewedImmunofluorescence images of nerve fiber and IL-31 receptors in the mouse ear skin
Project ID
386-Takahashi-EarIL31RA
Project SID
386
Title
Immunofluorescence images of nerve fiber and IL-31 receptors in the mouse ear skin
Description
IL-31 receptor blockade suppresses pruritus of atopic dermatitis. However, cell-type-specific contributions of IL-31 receptor to itch, its expression mechanism, and the downstream signaling pathway to induce itch remain unknown. In this report, using conditional knockout mice, the authors demonstrate that IL-31-induced itch requires sensory neuronal IL-31 receptor and STAT3. They find that IL-31 receptor expression is dependent on STAT3 in sensory neurons. In addition, pharmacological experiments suggest that STAT3 activation is important for the itch-inducing signaling downstream of the IL-31 receptor. A cutaneous IL-31 injection induces the nuclear accumulation of activated STAT3 first in sensory neurons that abundantly express IL-31 receptor and then in other itch-transmitting neurons. IL-31 enhances itch induced by various pruritogens including even chloroquine. Finally, pruritus associated with dermatitis is partially dependent on sensory neuronal IL-31 receptor and strongly on sensory neuronal STAT3. Thus, sensory neuronal STAT3 is essential for IL-31-induced itch and further contributes to IL-31-independent inflammatory itch.
Submission Date
2024-10-15
Opened Date
-
Release Date
2025-11-28
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
S.O. was supported by RIKEN Medical Sciences Innovation Hub Pro- gram (RIKEN MIH) and the Special Postdoctoral Researchers program at RIKEN. K.K. is supported by Japan Agency for Medical Research and Develop- ment (AMED) under CREST grant number JP23gm1210006. M.N. is supported by Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED under grant number JP21am0101119. K.S. is supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI grant 18H05411. T.O. was supported by AMED under grant number JP18ek0410033 and Japan Science and Technology Agency (JST) under CREST grant number JPMJCR17H1 and is supported by AMED under CREST grant number JP23gm1210006 and JST Moonshot R&D grant number JPMJMS2025.
Total
13 files / 16.9 GB
Image datasets
zipped 12 files / 13.4 GB, raw image 1 files / 3.5 GB, total 13 files / 16.9 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
B6.129-Scn10a^{tm2(cre)Jwo}/H,
B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J
(
https://www.infrafrontier.eu/emma/strain-details/?q=4582,
https://www.jax.org/strain/007914
)
B6.129-Scn10a^{tm2(cre)Jwo}/H,
B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J,
Il31ra-flox
(
https://www.infrafrontier.eu/emma/strain-details/?q=4582,
https://www.jax.org/strain/007914,
https://www.sciencedirect.com/science/article/pii/S2211124723014456?via%3Dihub
)
Cell
neuron,
keratinocyte
(
CL:0000312
)
neuron,
keratinocyte,
mesenchymal cell
(
CL:0008019
)
Cell line
-
MeSH
Ontology
Anatomical Entity
-
UBERON
skin of external ear
(
UBERON:0001459
)
Biological Process
-
Cellular Component
axon,
nucleus
(
GO:0005634
)
Molecular Function
-
Medical Subject Headings
Imaging Method
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
Paper DOI / Paper URL
People
Contact
Takaharu Okada
RIKEN IMS
Laboratory for Tissue Dynamics
-
-
Imaging dataset contributor
Sonoko Takahashi (RIKEN IMS)
Sotaro Ochiai (Malaghan Inst Med Res)
Noriko Takahashi (Kansai Med Univ)
Manabu Nakayama (Kazusa DNA Res Inst)
Takaharu Okada (RIKEN IMS)
Quantitative dataset contributor
-
Image datasets
10
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Z-series immunofluorescence images of the ear skin from control mouse
Z-series immunofluorescence images of the ear skin from control mouse
Z-series immunofluorescence images of the ear skin from control mouse
Z-series immunofluorescence images of the ear skin from control mouse
Z-series immunofluorescence images of the ear skin from control mouse
Z-series immunofluorescence images of the ear skin from control mouse
Z-series immunofluorescence images of the ear skin from control mouse
Z-series immunofluorescence images of the ear skin from IL-31 RA-deleted mouse
Z-series immunofluorescence images of the ear skin from IL-31 RA-deleted mouse
Z-series immunofluorescence images of the ear skin from IL-31 RA-deleted mouse