380-Wang-ActininTensionSensor
Author reviewedChanges of tension in smooth muscle cell after treatment with water, calyculin A, or Y27632
Project ID
380-Wang-ActininTensionSensor
Project SID
380
Title
Changes of tension in smooth muscle cell after treatment with water, calyculin A, or Y27632
Description
FRET-based sensors are utilized for real-time measurements of cellular tension. However, transfection of the sensor gene shows low efficacy and is only effective for a short period. Reporter mice expressing such sensors have been developed, but sensor fluorescence has not been measured successfully using conventional confocal microscopy. Therefore, methods for spatiotemporal measurement of cellular tension in vivo or ex vivo are still limited. The authors established a reporter mouse line expressing FRET-based actinin tension sensors consisting of EGFP as the donor and mCherry as the acceptor and whose FRET ratio change is observable with confocal microscopy. Tension-induced changes in FRET signals were monitored in the aorta and tail tendon fascicles, as well as aortic smooth muscle cells isolated from these mice. The pattern of FRET changes was distinctive, depending on tissue type. Indeed, aortic smooth muscle cells exhibit different sensitivity to macroscopic tensile strain in situ and in an isolated state. This mouse strain will enable novel types of biomechanical investigations of cell functions in important physiological events.
Submission Date
2024-08-23
Opened Date
-
Release Date
2024-12-14
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
AMED-CREST from the Japan Agency for Medical Research and Development (JP19gm0810005), the NAKATANI Foundation, and JSPS KAKENHIs (Nos. 21H04533 and 21K19902)モンダイ
Total
7 files / 5.9 GB
Image datasets
zipped 3 files / 2.5 GB, raw image 4 files / 3.4 GB, total 7 files / 5.9 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
R26R-S2 (Accession No. CDB0055E)
(
https://large.riken.jp/distribution/reporter-mouse.html
)
Cell
vascular associated smooth muscle cell
(
CL:0000359
)
Cell line
-
MeSH
Muscle, Smooth, Vascular
(
D031541, D018613, D059008
)
Muscle, Smooth, Vascular
Osmotic Pressure
Cytoskeleton
Ontology
Anatomical Entity
-
UBERON
blood vessel smooth muscle
(
UBERON:0004237
)
Biological Process
response to osmotic stress
(
GO:0006970
)
response to chemical
(
GO:0042221
)
Cellular Component
cytoskeleton
(
GO:0005856
)
Molecular Function
-
Medical Subject Headings
Muscle, Smooth, Vascular
(
D031541, D018613, D059008
)
Muscle, Smooth, Vascular
Osmotic Pressure
Cytoskeleton
Imaging Method
Method involved in biological imaging
FRET
(
FBbi:00000367
)
confocal microscopy
(
FBbi:00000251
)
time lapse microscopy
(
FBbi:00000249
)
Paper DOI / Paper URL
People
Contact
Takeo Matsumoto
Nagoya University
Biomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering
-
Furo-Cho, Chikusa-Ku, Nagoya, Aichi 464-8603, Japan
Imaging dataset contributor
Junfeng Wang (Nagoya University)
Yuki Tsujimura (RIKEN)
Takaya Abe (RIKEN)
Hiroshi Kiyonari (RIKEN)
Tetsuya Kitaguchi (Tokyo Institute of Technology)
Quantitative dataset contributor
-
Image datasets
3
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of tension sensor in VSMCs isolated from the aorta before and after stimulation with distilled water
Time-lapse images of tension sensor in VSMCs isolated from the aorta before and after stimulation with calyculin A
Time-lapse images of tension sensor in VSMCs isolated from the aorta before and after stimulation with Y27632サクジョ