SSBD:database v3

380-Wang-ActininTensionSensor

OMERO gallery,

OMERO index

Project

Title

Changes of tension in smooth muscle cell after treatment with water, calyculin A, or Y27632

Description

FRET-based sensors are utilized for real-time measurements of cellular tension. However, transfection of the sensor gene shows low efficacy and is only effective for a short period. Reporter mice expressing such sensors have been developed, but sensor fluorescence has not been measured successfully using conventional confocal microscopy. Therefore, methods for spatiotemporal measurement of cellular tension in vivo or ex vivo are still limited. The authors established a reporter mouse line expressing FRET-based actinin tension sensors consisting of EGFP as the donor and mCherry as the acceptor and whose FRET ratio change is observable with confocal microscopy. Tension-induced changes in FRET signals were monitored in the aorta and tail tendon fascicles, as well as aortic smooth muscle cells isolated from these mice. The pattern of FRET changes was distinctive, depending on tissue type. Indeed, aortic smooth muscle cells exhibit different sensitivity to macroscopic tensile strain in situ and in an isolated state. This mouse strain will enable novel types of biomechanical investigations of cell functions in important physiological events.

License

CC BY

Released at

Oct. 20, 2025, midnight

Updated at

Funding

AMED-CREST from the Japan Agency for Medical Research and Development (JP19gm0810005), the NAKATANI Foundation, and JSPS KAKENHIs (Nos. 21H04533 and 21K19902)

# of image datasets

3

Papers

Papers

Paper information: Wang J, Maeda E, Tsujimura Y, Abe T, Kiyonari H, Kitaguchi T, Yokota H, Matsumoto T. In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor. Sci Rep. 2023 Dec 20;13(1):22729.,
Pubmed ID: 38123655,
PMCID: PMC10733408,
DOI: https://doi.org/10.1038/s41598-023-50142-z,
URL: https://pubmed.ncbi.nlm.nih.gov/38123655/

Contacts

Contacts

Takeo Matsumoto (Contact) , Biomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering , Nagoya University
Junfeng Wang (Imaging Contributor) , Nagoya University
Yuki Tsujimura (Imaging Contributor) , RIKEN
Takaya Abe (Imaging Contributor) , RIKEN
Hiroshi Kiyonari (Imaging Contributor) , RIKEN
Tetsuya Kitaguchi (Imaging Contributor) , Tokyo Institute of Technology

Biosamples

Organisms

Mus musculus (NCBI:txid10090)

Strains

R26R-S2 (Accession No. CDB0055E)

Cells

vascular associated smooth muscle cell (CL_0000359)

Cell lines

Ontologies

UBERON

blood vessel smooth muscle (UBERON_0004237)