377-Ichinose-RedAlgae
Author reviewedBeta-tubulin dynamics during cell cycles of red algae
Project ID
377-Ichinose-RedAlgae
Project SID
377
Title
Beta-tubulin dynamics during cell cycles of red algae
Description
Live cell imaging by fluorescence microscopy is a useful tool for elucidating the localization and function of proteins and organelles in single cells. Especially, time-lapse analysis observing the same field sequentially can be used to observe cells of many organisms and analyze the dynamics of intracellular molecules. By single-cell analysis, it is possible to elucidate the characteristics and fluctuations of individual cells, which cannot be elucidated from the data obtained by averaging the characteristics of an ensemble of cells. The primitive red alga Cyanidioschyzon merolae has a very simple structure and is considered a useful model organism for studying the mechanism of organelle division, since the division is performed synchronously with the cell cycle. However, C. merolae does not have a rigid cell wall, and environmental changes such as low temperature or high pH cause morphological change and disruption easily. Therefore, morphological studies of C. merolae typically use fixed cells. In this study, The authors constructed a long-term time-lapse observation system to analyze the dynamics of proteins in living C. merolae cells. From the results, they elucidate the cell division process of single living cells, including the function of intracellular components.
Submission Date
2024-08-07
Opened Date
-
Release Date
2025-01-31
Update Date
-
Kind
Image dataset
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
This study received funds from Grant-in-Aid for Exploratory Research and Grant-Aid for Scientific Research (B).
Total
466 files / 1.4 GB
Image datasets
zipped 1 files / 508.9 MB, raw image 465 files / 930.2 MB, total 466 files / 1.4 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Cyanidioschyzon merolae
(
NCBITaxon:45157
)
Strain
Cell
red algae (Rhodophyta)
(
https://www.ncbi.nlm.nih.gov/datasets/taxonomy/2763/
)
Cell line
-
MeSH
Cell Cycle,
Cell Division
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
cell cycle (GO:0007049), cell division
(
GO:0051301
)
Cellular Component
-
Molecular Function
tubulin binding
(
GO:0015631
)
Medical Subject Headings
Cell Cycle,
Cell Division
Imaging Method
Method involved in biological imaging
fluorescence microscopy
(
FBbi:00000246
)
Paper DOI / Paper URL
People
Contact
Atsuko H Iwane
RIKEN BDR
Laboratory for Cell Field Structure
-
3-10-23, Kagamiyama, Higashi-hiroshima, Hiroshima, 739-0046, Japan
Imaging dataset contributor
Takako M Ichinose
Quantitative dataset contributor
-
Image datasets
1
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of Cyanidioschyzon merolae cells stably expressing beta-tubulin-sfGFP