306-Kamei-microglia
Author reviewedIn vivo imaging of the phagocytic dynamics underlying efficient clearance of adult-born hippocampal granule cells by ramified microglia
Project ID
306-Kamei-microglia
Project SID
306
Title
In vivo imaging of the phagocytic dynamics underlying efficient clearance of adult-born hippocampal granule cells by ramified microglia
Description
The phagocytosis of dead cells by microglia is essential in brain development and homeostasis. However, the mechanism underlying the efficient removal of cell corpses by ramified microglia remains poorly understood. Here, the authors investigated the phagocytosis of dead cells by ramified microglia in the hippocampal dentate gyrus, where adult neurogenesis and homeostatic cell clearance occur. Two-color imaging of microglia and apoptotic newborn neurons revealed two important characteristics. Firstly, frequent environmental surveillance and rapid engulfment reduced the time required for dead cell clearance. The motile microglial processes frequently contacted and enwrapped apoptotic neurons at the protrusion tips and completely digested them within 3–6 h of the initial contact. Secondly, while a single microglial process engaged in phagocytosis, the remaining processes continued environmental surveil- lance and initiated the removal of other dead cells. The simultaneous removal of multiple dead cells increases the clearance capacity of a single microglial cell.
Submission Date
2024-03-11
Opened Date
-
Release Date
2024-12-14
Update Date
-
Kind
Image datasets and Quantitative datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
Japan Agency for Medical Research and Development. Grant Numbers: JP19gm1310003, JP22jm0210097, Ministry of Education, Culture, Sports, Science and Technology. Grant Numbers: 20H00481, 20H05894, 20H05895
Total
40 files / 98.1 GB
Image datasets
zipped 20 files / 28.1 GB, raw image 20 files / 70.0 GB, total 40 files / 98.1 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
CX3CR1^{+/GFP}, Nes-CreER^{T2+/-}, Rosa-CAG-LSL- tdTomato^{+/-}
(
https://www.jax.org/strain/005582#, https://www.jax.org/strain/007909
)
Cell
neuron, microglial cell
(
CL:0000129
)
Cell line
-
MeSH
Neurons, Neuroglia
(
D059008
)
Ontology
Anatomical Entity
-
UBERON
brain
(
UBERON:0000955
)
Biological Process
phagocytosis
(
GO:0006909
)
phagocytosis, engulfment
(
GO:0006911
)
Cellular Component
-
Molecular Function
-
Medical Subject Headings
Neurons, Neuroglia
(
D059008
)
Imaging Method
Method involved in biological imaging
two-photon laser scanning microscopy
(
FBbi:00000254
)
time lapse microscopy
(
FBbi:00000249
)
Paper DOI / Paper URL
People
Contact
Shigeo Okabe
University of Tokyo
Department of Cellular Neurobiology, Graduate School of Medicine
-
-
Imaging dataset contributor
Ryosuke Kamei (University of Tokyo)
Quantitative dataset contributor
-
Image datasets
20
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse two-photon images of phagocytic events in the subgranular zone of mouse hippocampus
Time-lapse in vivo two-photon images of tdTomato-positive newborn neurons and GFP-positive microglia
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of phagocytic events until phagosomal content in the microglial processes was digested
Time-lapse two-photon images of retruction of microglial processes having phagosome
Time-lapse two-photon images of retruction of microglial processes having phagosome
Time-lapse two-photon images of retruction of microglial processes having phagosome
Time-lapse two-photon images of retruction of microglial processes having phagosome
Time-lapse two-photon images of retruction of microglial processes having phagosome
Time-lapse in vivo images of one microglial cell with multiple phagocytic events