Dataset Fig4_AF_Neopetromin
Project
451-Hitora-Neopetromin
Title
The shape of vacuole and F-actin in tobacco BY-2 cells treated with neopetromin, a newly found peptide
Description
HPLC-MS analysis revealed the presence of an
unreported peptide in the extract of the marine sponge Neopetrosia
sp. Its structure was determined as a tripeptide, named
neopetromin (1), composed of two tyrosine and one tryptophan
residues with a heteroaromatic C−N cross-link between side
chains. The absolute configuration of amino acids was determined
using Marfey’s method after ozonolysis and hydrolysis of 1.
Compound 1 promoted vacuole fragmentation in an actin-
independent manner in tobacco BY-2 cells.
Funding
This work was supported by JSPS KAKENHI Grant Number
26305005 (S.T.) and the Sumitomo Foundation Grant
Number 2330082 (T.H.).
Title
Z-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the presence of neopetromin
Description
Z-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the presence of neopetromin. fABD2 is C-terminal half of AtFim1 (aa 325–687), which includes the second actin-binding domain. Neopetromin is a newly found peptide from the Marine Sponge Neopetrosia sp.
Description
Tobacco BY-2 cells stably expressing GFP-fABD2 that was treated with neopetromin.
Extrinsic variables
Neopetromin (10 micromolar) treatment for 5 days
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Probes
macromolecular probe,
fABD2 which binds to F-actin (
FBbi:00000410)
Dimensions
731x957x60x1x1
X scales
0.185 micrometer
Y scales
0.185 micrometer
Visualization methods
Green fluorescent protein from Aequorea
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods
living tissue
Body
Olympus-Evident IX70
Detector
Prime
95B; Teledyne Photometrics, Tucson, AZ, USA
Objective
Olympus-Evident UPLSAP60XS2