SSBD Dataset Detail

Description

Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop- equipped SPIM for cleared specimens), a low-cost ($20,000–50,000), low- expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three- dimensional manner. Academically open-sourced (https://github.com/dbsb- juntendo/descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.

Funding

This study was supported by the Japan Agency for Medical Research and Development (AMED) PRIME (to E.A.S., grant number JP20gm6210027); AMED Research on Development of New Drugs (to A.H. and E.A.S., grant number JP21ak0101181); AMED Brain/MINDS (to E.A.S. and T.Nemoto, grant number JP21wm0425003, and JP19dm0207078); AMED Project for Promotion of Cancer Research and Therapeutic Evolution (P-PROMOTE) (to E.A.S., grant number JP22ama221517); Japan Science and Technology Agency (JST) CREST (to K.Otomo and E.A.S., grant number JPMJCR20E4 and JPMJCR23B7); Japan Society for the Promotion of Science (JSPS) KAKENHI grant-in-aid for scientific research (B) (to K.Otomo and E.A.S., grant number JP22H02756 and JP22H02824); JSPS KAKENHI grant-in-aid for scientific research (S) (to T.Nemoto and K.Otomo, grant number JP20H05669); JSPS KAKENHI grant-in-aid for challenging research (exploratory) (to K.Otomo, grant number JP21K19346); JSPS KAKENHI grant-in-aid for transformative research areas - platforms for advanced technologies and research resources “Advanced Bioimaging Support” (to T.Nemoto and E.A.S., grant number JP22H04926); JSPS KAKENHI grant-in-aid for fund for the promotion of joint international research (Fostering Joint International Research(B)) (to K.Otomo, grant number JP22KK0100); JSPS KAKENHI for international leading research (to E.A.S., grant number JP23K20044); Operating Costs Subsidies for Private Universities (to E.A.S.); Grants-in-Aid from UTEC-UTokyo (to E.A.S.); the Takeda Science Foundation (to E.A.S.); Nakatani foundation for advancement of measuring technologies in biomedical engineering (to E.A.S.); The Mochida Memorial Foundation for Medical and Pharmaceutical Research (to E.A.S.), and The Uehara Memorial Foundation (to E.A.S.).

Title

Z-series images of a BT-474 cell line-derived xenograft labeled with DyLight 650-Trastuzumab, PI, and CD31

Description

Z-series images of a xenograft derived from BT-474 human breast cancer cell line. The tumor xenograft was labeled with DyLight 650-Trastuzumab, propidium iodide, and CD31. The imaging was conducted with descSPIM, the desktop- equipped SPIM (selective plane illumination microscopy). The light sheet focus is 500 um left side of the xenograft along the X axis. This dataset is the result (angle1) of another result of the dataset (angle2), Fig4_xenograft_plus500_1 , in which the images were measured from the oppsite side.

Description

BT-474 human breast cancer cells suspended in PBS and equal volume of Matrigel was subcutaneously injected into the flank of the 5 to 6-week-old female SCID-beige mouse. FITC-labeled anti-murine CD31 antibody and the DyLightTM 650-labeled Trastuzumab were intravenously administered from the tail vein 24 h before sacrifice. The xenograft was excised, fixed, cleared, and stained.

SSBD:database v3

Dataset Fig4_xenograft_plus500_2

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