Dataset Fig3_brain_LeftAnterior_1
Project
416-Otomo-descSPIM
Title
Images of brain and xenograft obtained by descSPIM, the desktop- equipped SPIM
Description
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop- equipped SPIM for cleared specimens), a low-cost ($20,000–50,000), low- expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three- dimensional manner. Academically open-sourced (https://github.com/dbsb- juntendo/descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.
Funding
This study was supported by the Japan Agency for Medical Research and Development (AMED) PRIME (to E.A.S., grant number JP20gm6210027); AMED Research on Development of New Drugs (to A.H. and E.A.S., grant number JP21ak0101181); AMED Brain/MINDS (to E.A.S. and T.Nemoto, grant number JP21wm0425003, and JP19dm0207078); AMED Project for Promotion of Cancer Research and Therapeutic Evolution (P-PROMOTE) (to E.A.S., grant number JP22ama221517); Japan Science and Technology Agency (JST) CREST (to K.Otomo and E.A.S., grant number JPMJCR20E4 and JPMJCR23B7); Japan Society for the Promotion of Science (JSPS) KAKENHI grant-in-aid for scientific research (B) (to K.Otomo and E.A.S., grant number JP22H02756 and JP22H02824); JSPS KAKENHI grant-in-aid for scientific research (S) (to T.Nemoto and K.Otomo, grant number JP20H05669); JSPS KAKENHI grant-in-aid for challenging research (exploratory) (to K.Otomo, grant number JP21K19346); JSPS KAKENHI grant-in-aid for transformative research areas - platforms for advanced technologies and research resources “Advanced Bioimaging Support” (to T.Nemoto and E.A.S., grant number JP22H04926); JSPS KAKENHI grant-in-aid for fund for the promotion of joint international research (Fostering Joint International Research(B)) (to K.Otomo, grant number JP22KK0100); JSPS KAKENHI for international leading research (to E.A.S., grant number JP23K20044); Operating Costs Subsidies for Private Universities (to E.A.S.); Grants-in-Aid from UTEC-UTokyo (to E.A.S.); the Takeda Science Foundation (to E.A.S.); Nakatani foundation for advancement of measuring technologies in biomedical engineering (to E.A.S.); The Mochida Memorial Foundation for Medical and Pharmaceutical Research (to E.A.S.), and The Uehara Memorial Foundation (to E.A.S.).
Title
Z-series descSPIM images of Thy1-YFP-H mouse brain stained with propidium iodide
Description
Z-series images of Thy1-YFP-H mouse brain stained with propidium iodide, which were obtained with descSPIM, the desktop- equipped SPIM (selective plane illumination
microscopy). This dataset contains the images of left and anterior region of the brain. The images were measured from ventral side of the brain.
Description
Thy1-YFP-H mouse brain fixed, cleared, and stained with propidium iodide
Intrinsic variables
Thy1-YFP-H transgenic mouse
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Dimensions
2160x4096x997x2x1
Microscopy types
fluorescence microscopy (
FBbi:00000246)
(selective plane illumination microscopy (SPIM))
Visualization methods
propidium iodide
YFP
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods
fixation method
Light source
Cobolt Skyra 488 nm and 561 nm
Detector
Thorlabs CMOS camera CS895MU
Objective
Thorlabs TL2X-SAP (NA = 0.1, WD= 56.3mm)
Filter set
Thorlabs, FBH520-40 and FBH600-40