Dataset Fig2F_C-uchidai-growth_1

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Project

Project
408-Sarper-TentacleArrange
Title
Z-series and time-lapse images showing phyllotaxis-like periodic and symmetric tentacle arrangements
Description
Periodic organ arrangements occur during growth and development and are widespread in both animals and plants. In bilaterian animals, repetitive organs are typically arranged periodically in a two-dimensional space defined by two body axes. In contrast, radially symmetrical animals and plants exhibit organ arrangements in three-dimensional space—around the body axis in animals and around the stem in plants. While the principles of periodic organ arrangement have been extensively studied in bilaterians, investigations in radially symmetrical animals remain limited. In the present study, the authors combined live imaging, quantitative analysis, and mathematical modeling to elucidate periodic organ arrangement in a radially symmetrical animal, Coryne uchidai (Cnidaria, Hydrozoa). The polyps of C. uchidai simultaneously formed multiple tentacles in a regularly spaced, ring-like pattern exhibiting radial symmetry. Multiple such rings appeared periodically along the body and largely maintained symmetrical organization. Additionally, the researchers observed polymorphisms in symmetry types—specifically tri-, tetra-, and pentaradial symmetries—occurring as individual variations. Notably, the type of radial symmetry was positively correlated with polyp diameter, with pentaradial polyps exhibiting a larger diameter than tetra- or triradial ones. Their mathematical model suggested that the selection of size-dependent radial symmetry is governed by activation-inhibition dynamics and positional information emanating from the mouth that guides tentacle initiation.
License
CC BY
Funding
This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan for SS (22KJ3132) and KF (21K19297 and 22H04719).

Dataset

Title
Time-lapse images of the growing polyps of Coryne uchidai
Description
Time lapse images of Coryne uchidai in various stages including hydranth bud formation, transformation, tentacle initiation, and polyp growth. The Coryne uchidai was visualized with bright field observation.
License
CC BY
Submitted at
None
Released at
None
Updated at

Biosamples

Description
The living Coryne uchidai.
Organisms
Coryne uchidai (NCBI:txid662077)
Strains
Cells
Cell lines
Intrinsic variables
Extrinsic variables

Ontologies

UBERON
Anatomical entities
Gene ontology: Biological processes
growth (GO:0040007)
Gene ontology: Cellular components
Gene ontology: Molecular functions

Genetics

Gene names
Protein names
Genetic methods
Protein tags
Probes
Oligo Primer
Genotype

Treatments

Reagent or Compound
Concentrations
FoldDilution

Imaging Methods

Dimensions
1388x1040x211x1x106
X scales
2.53 micrometer
Y scales
2.53 micrometer
Z scales
15 micrometer
T scales
10 minute
Channels
1 channel
Microscopy types
time lapse microscopy (FBbi:00000249) confocal microscopy (FBbi:00000251)
Detection methods
Visualization methods
Illumination methods
Sources of contrast
Contrast enhancing methods
Resolution enhancing methods
Image parameters
Sample preparation methods
living tissue

Instruments

Body
Zeiss Axio Zoom.V16
Model
Light source
Detector
Objective
Filter set
Dichroic