Dataset Fig1M_WT1_Lobule3_2
Project
388-Okuno-CFinput
Title
Climbing fiber inputs to Purkinje cells in wild type mice and PTPdelta knockout mice
Description
Functionally mature neural circuits are shaped during postnatal development
by eliminating redundant synapses formed during the perinatal period. In the
cerebellum of neonatal rodents, each Purkinje cell (PC) receives synaptic inputs
from multiple (more than 4) climbing fibers (CFs). During the first 3 postnatal
weeks, synaptic inputs from a single CF become markedly larger and those from
the other CFs are eliminated in each PC, leading to mono-innervation of each PC
by a strong CF in adulthood. While molecules involved in the strengthening and
elimination of CF synapses during postnatal development are being elucidated,
much less is known about the molecular mechanisms underlying CF synapse
formation during the early postnatal period. The authors show experimental evidence
that suggests that a synapse organizer, PTPdelta, is required for early postnatal CF
synapse formation and the subsequent establishment of CF to PC synaptic wiring.
They showed that PTPdelta was localized at CF-PC synapses from postnatal day 0
(P0) irrespective of the expression of Aldolase C (Aldoc), a major marker of PC
that distinguishes the cerebellar compartments. They found that the extension of
a single strong CF along PC dendrites (CF translocation) was impaired in global
PTPdelta knockout (KO) mice from P12 to P29-31 predominantly in PCs that did
not express Aldoc [Aldoc (–) PCs].
Title
Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse
Description
Z-series images of synaptic inputs to the cerebellar lobule3 in wild type mouse. Aldoc is not expressed in the Purkinje cells at the cerebellar lobule3. Channel1; Car8, Channel2; Aldoc, Channel3; VGluT2
Description
Fixed brain slice from wild type P12 mouse
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Protein names
Carbonic anhydrase-related protein (
P28651)
Fructose-bisphosphate aldolase C (
P05063)
Vesicular glutamate transporter 2 (
Q8BLE7)
Dimensions
1024x1024x20x3x1
Detection methods
photomultiplier tube (PMT)
Visualization methods
primary antibody plus labeled secondary antibody
Illumination methods
photomultiplier tube (PMT)
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods
fixation method
Body
Olympus-Evident FV1200
Objective
Olympus-Evident UPLSAPO40X