Dataset VideoS3_WithInfection_before
Project
344-Murata-InfectHeart
Title
Influence of persistent SARS-CoV-2 infection on a three- dimensional cardiac model
Description
Patients with chronic cardiomyopathy may have persistent viral infections in their hearts, particularly with SARS-CoV-2, which targets the ACE2 receptor highly expressed in human hearts. This raises concerns about a potential global heart failure pandemic stemming from COVID-19, an SARS-CoV-2 pandemic in near future. Although faced with this healthcare caveat, there is limited research on persistent viral heart infections, and no models have been established. In this study, we created an SARS-CoV-2 persistent infection model using human iPS cell-derived cardiac microtissues (CMTs). Mild infections sustained viral presence without signif- icant dysfunction for a month, indicating persistent infection. However, when exposed to hypoxic conditions mimicking ischemic heart diseases, cardiac function deteriorated alongside intracellular SARS-CoV-2 reacti- vation in cardiomyocytes and disrupted vascular network formation. This study demonstrates that SARS- CoV-2 persistently infects the heart opportunistically causing cardiac dysfunction triggered by detrimental stimuli such as ischemia, potentially predicting a post COVID-19 era heart failure pandemic.
Funding
Clinical Translational Research Program
Title
Time lapse images of pulsating cardiac microtissues with persistent infection of SARS-CoV-2 before hypoxia-reperfusion
Description
Time lapse images of pulsating cardiac microtissues with persistent infection of SARS-CoV-2 before hypoxia-reperfusion.
Description
Human iPS cell-derived cardiac microtissues. They are composed of cardiomyocytes and other cardiac component cells (vascular endothelial cells and vascular mural cells) differentiated from human iPS cells and yield a vascular network-like structure that morphologically and functionally mimics the human heart.
Cells
differentiated iPS cells (
)
Gene ontology: Biological processes
cardiac muscle contraction (GO:0060048), viral process (GO:0016032) (
GO:0016032)
Gene ontology: Cellular components
Gene ontology: Molecular functions
Detection methods
wide-field detection
Illumination methods
wide-field detection
Contrast enhancing methods
optical method
Resolution enhancing methods
Sample preparation methods
living tissue