SSBD:database v3

Project

Project

333-Tsugawa-gravitropism

Title

Shoot gravitropism of wild type and myosin xif xik mutant Arabidopsis

Description

Gravitropism is the plant organ bending in response to gravity, while a straightening mechanism prevents bending beyond the gravitropic set-point angle. The promotion and prevention of bending occur simultaneously around the inflorescence stem tip. How these two opposing forces work together and what part of the stem they affect are unknown. To understand the mechanical forces involved, authors rotated wild type and organ-straightening-deficient mutant (myosin xif xik) Arabidopsis plants to a horizontal position to initiate bending. The mutant stems started to bend before the wild-type stems, which led them to hypothesize that the force preventing bending was weaker in mutant.

License

CC BY

Funding

This work was supported by MEXT KAKENHI (nos. JP18H05484, JP18H05489 to TD and JP18H05496 to HU), by the Japan Society for the Promotion of Science [Grants-in-Aid for Scientific Research (no. JP20K15832 to ST, no. JP19K06732 to HU, and no. JP15H05776 to IH-N)] and by the Hirao Taro Foundation of KONAN GAKUEN for Academic Research (to HU and IH-N), by the Japan Science and Technology Agency [CREST (JPMJCR2121)].

Dataset

Title

Time-lapse images of shoot gravitropism for organ-straightening-deficient mutant (myosin xif xik) Arabidopsis

Description

Time-lapse images of shoot gravitropism for organ-straightening-deficient mutant (myosin xif xik) Arabidopsis. Photographs were taken by automatically every 10 min using a digital camera.

License

CC BY

Submitted at

None

Released at

None

Updated at

Biosamples

Description

Arabidopsis thaliana mutants that are defective in two myosin XI members (myosin XIf and myosin XIk)

Organisms

Arabidopsis thaliana (NCBI:txid3702)

Strains

Cells

Cell lines

Intrinsic variables

The T-DNA-tagged mutants myosin xif-1, and myosin xik-2.

Extrinsic variables

Ontologies

UBERON

Anatomical entities

Gene ontology: Biological processes

gravitropism (GO:0009630)

Gene ontology: Cellular components

Gene ontology: Molecular functions

Genetics

Gene names

NA

Protein names

Genetic methods

T-DNA insertion (classes?short_form=EFO_0004016)

Protein tags

Probes

Oligo Primer

Genotype

Treatments

Reagent or Compound

Concentrations

FoldDilution

Imaging Methods

Dimensions

588x464x1x1x38

X scales

0.151 micrometer/pixel

Y scales

0.151 micrometer/pixel

Z scales

T scales

10 minutes

Channels

1 channel

Microscopy types

(macroscopy)

Detection methods

Visualization methods

Illumination methods

Sources of contrast

Contrast enhancing methods

Resolution enhancing methods

Image parameters

Sample preparation methods

living tissue

Instruments

Body

Model

Light source

Detector

Objective

Filter set

Dichroic