Dataset Fig1a_gravit_wild_8

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Project

Project
333-Tsugawa-gravitropism
Title
Shoot gravitropism of wild type and myosin xif xik mutant Arabidopsis
Description
Gravitropism is the plant organ bending in response to gravity, while a straightening mechanism prevents bending beyond the gravitropic set-point angle. The promotion and prevention of bending occur simultaneously around the inflorescence stem tip. How these two opposing forces work together and what part of the stem they affect are unknown. To understand the mechanical forces involved, authors rotated wild type and organ-straightening-deficient mutant (myosin xif xik) Arabidopsis plants to a horizontal position to initiate bending. The mutant stems started to bend before the wild-type stems, which led them to hypothesize that the force preventing bending was weaker in mutant.
License
CC BY
Funding
This work was supported by MEXT KAKENHI (nos. JP18H05484, JP18H05489 to TD and JP18H05496 to HU), by the Japan Society for the Promotion of Science [Grants-in-Aid for Scientific Research (no. JP20K15832 to ST, no. JP19K06732 to HU, and no. JP15H05776 to IH-N)] and by the Hirao Taro Foundation of KONAN GAKUEN for Academic Research (to HU and IH-N), by the Japan Science and Technology Agency [CREST (JPMJCR2121)].

Dataset

Title
Time-lapse images of shoot gravitropism for wild type Arabidopsis
Description
Time-lapse images of shoot gravitropism for wild type Arabidopsis. Photographs were taken by automatically every 10 min using a digital camera.
License
CC BY
Submitted at
None
Released at
None
Updated at

Biosamples

Description
Wild type Arabidopsis thaliana (accession Columbia-0 [CS60000])
Organisms
Arabidopsis thaliana (NCBI:txid3702)
Strains
Col-0
Cells
Cell lines
Intrinsic variables
Extrinsic variables

Ontologies

UBERON
Anatomical entities
Gene ontology: Biological processes
gravitropism (GO:0009630)
Gene ontology: Cellular components
Gene ontology: Molecular functions

Genetics

Gene names
Protein names
Genetic methods
Protein tags
Probes
Oligo Primer
Genotype

Treatments

Reagent or Compound
Concentrations
FoldDilution

Imaging Methods

Dimensions
544x504x1x1x38
X scales
0.152 micrometer/pixel
Y scales
0.152 micrometer/pixel
Z scales
T scales
10 minutes
Channels
1 channel
Microscopy types
(macroscopy)
Detection methods
Visualization methods
Illumination methods
Sources of contrast
Contrast enhancing methods
Resolution enhancing methods
Image parameters
Sample preparation methods
living tissue

Instruments

Body
Model
Light source
Detector
Objective
Filter set
Dichroic