We performed live imaging of the nuclear shape of the upper layer neurons of the visual cortex in young and aged NexCre;SUN1-GFP mice in vivo with two-photon microscopy before, during, and after visual light exposure following the prior preparations of craniotomy on the visual cortex for time-lapse imaging. NexCre;SUN1-GFP mice expressed the GFP-fused SUN1 protein, a protein on the outer nuclear membrane, in the excitatory neurons under the control of the NeuroD6/Nex gene locus.
Keywords; time-lapse imaging, two-photon microscopy, nucleus, excitatory neuron, visual cortex, visual stimulation, aging.
See detail in Frey et al., bioRxiv, 2022
Tanita Frey, Tomonari Murakami, Koichiro Maki, Takumi Kawaue, Ayaka Sugai, Naotaka Nakazawa, Taiji Adachi, Mineko Kengaku, Kenichi Ohki, Yukiko Gotoh, Yusuke Kishi (2022/01/01), Age-associated reduction of nuclear shape dynamics in excitatory neurons of the visual cortex, bioRxiv, 2022.08.22.504704
Published in 2022/01/01
(Abstract) None