Summary of ssbd-repos-000302

Name
URL
DOI

Title
Nuclear shape of excitatory neurons in the visual cortex upon visual stimulation of young and aged mice
Description

We performed live imaging of the nuclear shape of the upper layer neurons of the visual cortex in young and aged NexCre;SUN1-GFP mice in vivo with two-photon microscopy before, during, and after visual light exposure following the prior preparations of craniotomy on the visual cortex for time-lapse imaging. NexCre;SUN1-GFP mice expressed the GFP-fused SUN1 protein, a protein on the outer nuclear membrane, in the excitatory neurons under the control of the NeuroD6/Nex gene locus.
Keywords; time-lapse imaging, two-photon microscopy, nucleus, excitatory neuron, visual cortex, visual stimulation, aging.

Submited Date
2023-06-09
Release Date
2023-06-09
Updated Date
-
License
Funding information
-
File formats
TIFF, Nikon NIS-Elements ND2 Image Format (.nd2)
Data size
2.5 GB

Organism
Mus Musculus
Strain
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Cell Line
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Genes
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Proteins
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GO Molecular Function (MF)
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GO Biological Process (BP)
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GO Cellular Component (CC)
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Study Type
-
Imaging Methods
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Method Summary

See detail in Frey et al., bioRxiv, 2022

Related paper(s)

Tanita Frey, Tomonari Murakami, Koichiro Maki, Takumi Kawaue, Ayaka Sugai, Naotaka Nakazawa, Taiji Adachi, Mineko Kengaku, Kenichi Ohki, Yukiko Gotoh, Yusuke Kishi (2022/01/01), Age-associated reduction of nuclear shape dynamics in excitatory neurons of the visual cortex, bioRxiv, 2022.08.22.504704

Published in 2022/01/01

(Abstract) None

Contact(s)
Yusuke Kishi
Organization(s)
The University of Tokyo , Institute for Quantitative Biosciences , Laboratory of Molecular Neurobiology
Image Data Contributors
Tanita Frey
Quantitative Data Contributors

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