|Summary of ssbd-repos-000174|
|Title||Redundant and specific roles of EGFR ligands in the ERK activation waves during collective cell migration of MDCK cells|
Epidermal growth factor receptor (EGFR) plays a pivotal role in collective cell migration by mediating cell-to-cell propagation of extracellular signal-regulated kinase (ERK) activation. Here, we aimed to determine which EGFR ligands mediate the ERK activation waves by gene knockout. Four of the seven known EGFR ligands are expressed in MDCK cells. We found that epidermal growth factor (EGF)-deficient cells exhibited lower basal ERK activity than the cells deficient in heparin-binding EGF (HBEGF), transforming growth factor alpha (TGFα) or epiregulin (EREG), but all cell lines deficient in a single EGFR ligand retained the ERK activation waves. Therefore, we knocked out the EGFR ligand genes in decreasing order of expression. ERK activation waves were markedly suppressed, albeit incompletely, only when all four EGFR ligands were knocked-out. Re-expression of the EGFR ligands revealed that all but HBEGF could restore the ERK activation waves. Aiming at complete elimination of the ERK activation waves, we further attempted to knockout Nrg1, a ligand for ErbB3 and ErbB4, and found that Nrg1 deficiency induced growth arrest in the absence of all four EGFR ligand genes expressed in MDCK cells. Collectively, these results showed that EGFR ligands exhibit remarkable redundancy in the propagation of ERK activation waves during collective cell migration.
|License||CC BY-NC license, https://creativecommons.org/licenses/by-nc/4.0/||Contact Information|
|Organization(s)||Graduate School of Biostudies, Kyoto University , Research Center for Dynamic Living Systems , Laboratory of Bioimaging and Cell Signaling|
|Image Data Contributors||Shuhao Lin|
|Quantitative Data Contributors||Shuhao Lin, Michiyuki Matsuda||Method Information|
See details in Lin S, Hirayama D, Maryu G, Matsuda K, Hino N, Deguchi E, Aoki K, Iwamoto R, Terai K, Matsuda M. Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration. Life Sci Alliance. 2021 Oct 19;5(1):e202101206. doi: 10.26508/lsa.202101206. PMID: 34667080.
|Related Paper||doi:10.26508/lsa.202101206 , PMID:34667080 , https://www.life-science-alliance.org/content/5/1/e202101206|
|Paper information||Lin S, Hirayama D, Maryu G, Matsuda K, Hino N, Deguchi E, Aoki K, Iwamoto R, Terai K, Matsuda M. Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration. Life Sci Alliance. 2021 Oct 19;5(1):e202101206. doi: 10.26508/lsa.202101206. PMID: 34667080.|
|Date of publication||Oct. 19, 2021||Dataset Information|
|Data size||90.3 GB|
|Cell line||MDCK cells: RIKEN BioResource Center (no. RCB0995)|
|Molecular Function (MF)||epidermal growth factor|
|Biological Process (BP)||cell migration|
|Cellular Component (CC)||plasma membrane, extracellular space|
|Study Type||Cell Culture Techniques|
|Imaging Methods||Fluorescence Microscopy|
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