SSBD:repository

Summary of ssbd-repos-000174
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Repository URL https://ssbd.riken.jp/repository/174/
DOI https://doi.org/10.24631/ssbd.repos.2021.05.002
Title Redundant and specific roles of EGFR ligands in the ERK activation waves during collective cell migration of MDCK cells
Description

Epidermal growth factor receptor (EGFR) plays a pivotal role in collective cell migration by mediating cell-to-cell propagation of extracellular signal-regulated kinase (ERK) activation. Here, we aimed to determine which EGFR ligands mediate the ERK activation waves by gene knockout. Four of the seven known EGFR ligands are expressed in MDCK cells. We found that epidermal growth factor (EGF)-deficient cells exhibited lower basal ERK activity than the cells deficient in heparin-binding EGF (HBEGF), transforming growth factor alpha (TGFα) or epiregulin (EREG), but all cell lines deficient in a single EGFR ligand retained the ERK activation waves. Therefore, we knocked out the EGFR ligand genes in decreasing order of expression. ERK activation waves were markedly suppressed, albeit incompletely, only when all four EGFR ligands were knocked-out. Re-expression of the EGFR ligands revealed that all but HBEGF could restore the ERK activation waves. Aiming at complete elimination of the ERK activation waves, we further attempted to knockout Nrg1, a ligand for ErbB3 and ErbB4, and found that Nrg1 deficiency induced growth arrest in the absence of all four EGFR ligand genes expressed in MDCK cells. Collectively, these results showed that EGFR ligands exhibit remarkable redundancy in the propagation of ERK activation waves during collective cell migration.

Submited Date 2021-05-22
Release Date 2021-10-18
Updated Date -
License CC BY-NC license, https://creativecommons.org/licenses/by-nc/4.0/
Contact Information
Contact(s) Michiyuki Matsuda
Organization(s) Graduate School of Biostudies, Kyoto University , Research Center for Dynamic Living Systems , Laboratory of Bioimaging and Cell Signaling
Image Data Contributors Shuhao Lin
Quantitative Data Contributors Shuhao Lin, Michiyuki Matsuda
Method Information
Method Summary

See details in Lin S, Hirayama D, Maryu G, Matsuda K, Hino N, Deguchi E, Aoki K, Iwamoto R, Terai K, Matsuda M. Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration. Life Sci Alliance. 2021 Oct 19;5(1):e202101206. doi: 10.26508/lsa.202101206. PMID: 34667080.

Related Paper doi:10.26508/lsa.202101206 , PMID:34667080 , https://www.life-science-alliance.org/content/5/1/e202101206
Paper information Lin S, Hirayama D, Maryu G, Matsuda K, Hino N, Deguchi E, Aoki K, Iwamoto R, Terai K, Matsuda M. Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration. Life Sci Alliance. 2021 Oct 19;5(1):e202101206. doi: 10.26508/lsa.202101206. PMID: 34667080.
Date of publication Oct. 19, 2021
Dataset Information
Data size 90.3 GB
File formats TIF
Organism canis familiaris
Strain -
Cell line MDCK cells: RIKEN BioResource Center (no. RCB0995)
Molecular Function (MF) epidermal growth factor
Biological Process (BP) cell migration
Cellular Component (CC) plasma membrane, extracellular space
Study Type Cell Culture Techniques
Imaging Methods Fluorescence Microscopy

Datasets

If .oif.zip file exists, download .oif.zip file. It is an archive containing .oif and .oif.files/.


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