Summary of ssbd-repos-000169

Repository URL
Sets of image data of mouse mitral cells genetically labeled with utero electroporation.

Morphology and FRET/Ca2+ imaging data of mitral cells in the mouse olfactory bulb.

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Release Date
Updated Date
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162.2 GB
Data formats
tif, lif, oib

Mus musculus
Cell Line
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Study Type
Imaging Methods

Method Summary

See detail in Aihara S, Fujimoto S, Sakaguchi R, Imai T. BMPR-2 gates activity-dependent stabilization of primary dendrites during mitral cell remodeling. Cell Rep. 2021 Jun 22;35(12):109276. doi: 10.1016/j.celrep.2021.109276. PMID: 34161760.

Related paper(s)

Shuhei Aihara, Satoshi Fujimoto, Richi Sakaguchi, Takeshi Imai (2021) BMPR-2 gates activity-dependent stabilization of primary dendrites during mitral cell remodeling., Cell reports, Volume 35, Number 12, pp. 109276

Published in 2021 Jun 22

(Abstract) Developing neurons initially form excessive neurites and then remodel them based on molecular cues and neuronal activity. Developing mitral cells in the olfactory bulb initially extend multiple primary dendrites. They then stabilize single primary dendrites while eliminating others. However, the mechanisms underlying selective dendrite remodeling remain elusive. Using CRISPR-Cas9-based knockout screening combined with in utero electroporation, we identify BMPR-2 as a key regulator for selective dendrite stabilization. Bmpr2 knockout and its rescue experiments show that BMPR-2 inhibits LIMK without ligands and thereby permits dendrite destabilization. In contrast, the overexpression of antagonists and agonists indicates that ligand-bound BMPR-2 stabilizes dendrites, most likely by releasing LIMK. Using genetic and FRET imaging experiments, we demonstrate that free LIMK is activated by NMDARs via Rac1, facilitating dendrite stabilization through F-actin formation. Thus, the selective stabilization of primary dendrites is ensured by concomitant inputs of BMP ligands and neuronal activity.
(MeSH Terms)

Takashi Imai
Kyushu University , Department of Developmental Neurophysiology, Graduate School of Medical Sciences
Image Data Contributors
Shuhei Aihara, Satoshi Fujimoto, Richi Sakaguchi, Takashi Imai
Quantitative Data Contributors

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