Raw data of an article “Multiple types of navigational information are diffusely and independently encoded in the population activities of the dentate gyrus neurons” (Murano et al., 2020)
Description
The dentate gyrus (DG) plays critical roles in cognitive functions such as learning, memory, and spatial coding, and its dysfunction is implicated in various neuropsychiatric disorders. However, it remains largely unknown how information is represented in this region. Here, we recorded neuronal activity in the DG using Ca2+ imaging in freely moving mice and analysed this activity using machine learning. Although each individual neuron was weakly and diversely tuned to multiple information types, the activity patterns of populations of DG neurons enabled us to successfully decode position, speed, and motion direction in an open field as well as current and future location in a T-maze. In αCaMKII heterozygous knockout mice, an animal model of neuropsychiatric disorders, including intellectual disability and bipolar disorder, the decoding accuracy of position in the open field and future location in the T-maze were selectively reduced. These results suggest that multiple types of information are diffusely and independently distributed in DG neurons.
Raw and processed calcium fluorescence data in the original format of nVista system (inscopix; *.isxd files, paired with *.isxp metafile), count of active cell population (*.xlsx), extracted calcium signal traces of each neuron (*.csv), and cell countour images (*.tiff) are registered. The [*.isxd] data stored in a folder (*_data) are linked to a metafile (*.isxp) located in their parent folder, and these files can be opened with “Inscopix Data Processing Software‘’ available from inscopix (https://www.inscopix.com/). Processed datasets and codes used for the decoding analyses are available at GitHub (https://github.com/tmurano).
Data size
4.9 TB
Organism
Mus musculus
Strain
C57BL/6J
Cell Line
NA
Genes
-
Proteins
-
GO Molecular Function (MF)
The mutant grpoup of the mice which we studied have heterozygous knockout of a gene coding calcium/calmodulin dependent protein kinase II alpha (CaMKIIa) protein, whose function is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. These mutant mice is an animal model of neuropsychiatric disorders, including intellectual disability and bipolar disorder.
GO Biological Process (BP)
In the heterozygous CaMKIIa knockout mice, Calbindin, a marker of mature neurons in the dentate gyrus, is largely reduced. The neurons of these mutants had multiple features of normal immature neurons, at molecular, morphological and electrophysiological levels.
GO Cellular Component (CC)
NA
Study Type
in vivo, calcium imaging, navigational information, spatial coding, dematuration
See details in Murano T, Nakajima R, Nakao A, Hirata N, Amemori S, Murakami A, Kamitani Y, Yamamoto J, Miyakawa T. Multiple types of navigational information are independently encoded in the population activities of the dentate gyrus neurons. Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2106830119. doi: 10.1073/pnas.2106830119. Epub 2022 Aug 5. PMID: 35930667.
(See details in Murano T, et. al. (2020) BioRxiv. https://doi.org/10.1101/2020.06.09.141572 )