Two-photon calcium imaging of GCaMP3/6f expressed in olfactory sensory neurons or mitral/tufted cells in the mouse.
See details in Inagaki et al. (2020) Cell Rep., 31(13): 107814.
Shigenori Inagaki, Ryo Iwata, Masakazu Iwamoto, Takeshi Imai (2020) Widespread Inhibition, Antagonism, and Synergy in Mouse Olfactory Sensory Neurons In Vivo., Cell reports, Volume 31, Number 13, pp. 107814
Published in 2020 Jun 30
(Abstract) Sensory information is selectively or non-selectively enhanced and inhibited in the brain, but it remains unclear whether and how this occurs at the most peripheral level. Using in vivo calcium imaging of mouse olfactory bulb and olfactory epithelium in wild-type and mutant animals, we show that odors produce not only excitatory but also inhibitory responses in olfactory sensory neurons (OSNs). Heterologous assays indicate that odorants can act as agonists to some but inverse agonists to other odorant receptors. We also demonstrate that responses to odor mixtures are extensively suppressed or enhanced in OSNs. When high concentrations of odors are mixed, widespread antagonism suppresses the overall response amplitudes and density. In contrast, a mixture of low concentrations of odors often produces synergistic effects and boosts the faint odor inputs. Thus, odor responses are extensively tuned by inhibition, antagonism, and synergy at the most peripheral level, contributing to robust sensory representations.(MeSH Terms)
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