Detail of 20161027_Resille_sqhGFP_VF05_WT1



Project
Title
Time-lapse images of cell shape changes in the early stage of Drosophila ventral furrow formation
Description
Time-lapse images of cell shape changes in the early stage of Drosophila ventral furrow formation
Release, Updated
2020-12-23
License
CC BY
Kind
Image data based on Experiment related Quantitative data ; 20161027_Resille_sqhGFP_VF05_WT1
File Formats
TIFF
Data size
1.2 GB

Organism
Drosophila ( NCBI:txid7215 )
Strain(s)
-
Cell Line
-

Datatype
NA
Molecular Function (MF)
Biological Process (BP)
ventral furrow formation ( GO:0007370 ) mesodermal cell migration ( GO:0008078 )
Cellular Component (CC)
-
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
two-photon laser scanning microscopy ( Fbbi:00000254 )
X scale
0.0138889 centimeter/pixel
Y scale
0.0138889 centimeter/pixel
Z scale
1 centimeter/slice
T scale
10 sec per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in See details in Wen et al. (2017) Biophys J, 112(12): 2683-2695.
Related paper(s)

Fu-Lai Wen, Yu-Chiun Wang, Tatsuo Shibata (2017) Epithelial Folding Driven by Apical or Basal-Lateral Modulation: Geometric Features, Mechanical Inference, and Boundary Effects., Biophysical journal, Volume 112, Number 12, pp. 2683-2695

Published in 2017 Jun 20

(Abstract) During embryonic development, epithelial sheets fold into complex structures required for tissue and organ functions. Although substantial efforts have been devoted to identifying molecular mechanisms underlying epithelial folding, far less is understood about how forces deform individual cells to sculpt the overall sheet morphology. Here we describe a simple and general theoretical model for the autonomous folding of monolayered epithelial sheets. We show that active modulation of intracellular mechanics along the basal-lateral as well as the apical surfaces is capable of inducing fold formation in the absence of buckling instability. Apical modulation sculpts epithelia into shallow and V-shaped folds, whereas basal-lateral modulation generates deep and U-shaped folds. These characteristic tissue shapes remain unchanged when subject to mechanical perturbations from the surroundings, illustrating that the autonomous folding is robust against environmental variabilities. At the cellular scale, how cells change shape depends on their initial aspect ratios and the modulation mechanisms. Such cell deformation characteristics are verified via experimental measurements for a canonical folding process driven by apical modulation, indicating that our theory could be used to infer the underlying folding mechanisms based on experimental data. The mechanical principles revealed in our model could potentially guide future studies on epithelial folding in diverse systems.
(MeSH Terms)

Contact
Tatsuo Shibata, Fu-Lai Wen , RIKEN, RIKEN , Center for Biosystems Dynamics Research, Center for Biosystems Dynamics Research , Laboratory for Physical Biology, Laboratory for Physical Biology
Contributors
NA

OMERO Dataset
OMERO Project
Source