Summary of ssbd-repos-000418

Name
URL
DOI

Title
Development of DGLa expression in mouse visual cortex
Description

Immunoperoxidase staining of DGLa in the mouse visual cortex at various postnatal ages

Submited Date
2025-02-10
Release Date
2025-04-08
Updated Date
-
License
Funding information
-
File formats
tiff
Data size
272.3 MB

Organism
Mus musculus
Strain
C57BL/6J
Cell Line
NA
Genes
DAGLA
Proteins
diacylglycerol lipase, alpha

GO Molecular Function (MF)
protein binding, metal ion binding, monoacylglycerol lipase activity, lipoprotein lipase activity, hydrolase activity
GO Biological Process (BP)
cannabinoid biosynthetic process, retrograde trans-synaptic signaling by endocannabinoid, regulation of neuroinflammatory response, monoacylglycerol biosynthetic process, diacylglycerol catabolic process, arachidonate metabolic process, neurogenesis, neuroblast proliferation
GO Cellular Component (CC)
cytoplasm, postsynaptic membrane, postsynaptic density membrane, plasma membrane, early endosome membrane, varicosity, dendritic spine membrane, dendrite membrane
Study Type
NA
Imaging Methods
bright-field microscope

Method Summary

See details in Yoneda, et. al. (2024) iScience.

Related paper(s)

Taisuke Yoneda, Katsuro Kameyama, Takahiro Gotou, Keiko Terata, Masahiro Takagi, Yumiko Yoshimura, Kenji Sakimura, Masanobu Kano, Yoshio Hata (2024) Layer specific regulation of critical period timing and maturation of mouse visual cortex by endocannabinoids., iScience, Volume 27, Number 6, pp. 110145

Published in 2024 Jun 21 (Electronic publication in May 29, 2024, midnight )

(Abstract) Plasticity during the critical period is important for the functional maturation of cortical neurons. While characteristics of plasticity are diverse among cortical layers, it is unknown whether critical period timing is controlled by a common or unique molecular mechanism among them. We here clarified layer-specific regulation of the critical period timing of ocular dominance plasticity in the primary visual cortex. Mice lacking the endocannabinoid synthesis enzyme diacylglycerol lipase-alpha exhibited precocious critical period timing, earlier maturation of inhibitory synaptic function in layers 2/3 and 4, and impaired development of the binocular matching of orientation selectivity exclusively in layer 2/3. Activation of cannabinoid receptor restored ocular dominance plasticity at the normal critical period in layer 2/3. Suppression of GABA(A) receptor rescued precocious ocular dominance plasticity in layer 4. Therefore, endocannabinoids regulate critical period timing and maturation of visual function partly through the development of inhibitory synaptic functions in a layer-dependent manner.

Contact(s)
Yoshio Hata
Organization(s)
Tottori University , Division of Neuroscience, School of Life Science, Faculty of Medicine
Image Data Contributors
Taisuke Yoneda, Katsuro Kameyama
Quantitative Data Contributors

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