volumetric fluorescence imaging data of cardiac organoid derived from human-iPS cells, mouse brain section, and quail embryo
See detail in “Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo”, T. Ichimura, et al., eLife, DOI: 10.7554/elife.93633.1.
Taro Ichimura, Taishi Kakizuka, Yoshitsugu Taniguchi, Satoshi Ejima, Yuki Sato, Keiko Itano, Kaoru Seiriki, Hitoshi Hashimoto, Ko Sugawara, Hiroya Itoga, Shuichi Onami, Takeharu Nagai (2025) Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo., eLife, Volume 13
Published in 2025 Feb 3 (Electronic publication in Feb. 3, 2025, midnight )
(Abstract) We established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of x2 and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, we built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a transverse spatial resolution of approximately 1.1 microm across an FOV of approximately 1.5x1.0 cm(2). The 3D resolving capability was realized through a combination of optical and computational sectioning techniques tailored for our low-power imaging system. We applied the imaging technique to 1.2 cm-wide section of mouse brain, and successfully observed various regions of the brain with sub-cellular resolution in a single FOV. We also performed time-lapse imaging of a 1-cm-wide vascular network during quail embryo development for over 24 hr, visualizing the movement of over 4.0x10(5) vascular endothelial cells and quantitatively analyzing their dynamics. Our results demonstrate the potential of this technique in accelerating production of comprehensive reference maps of all cells in organisms and tissues, which contributes to understanding developmental processes, brain functions, and pathogenesis of disease, as well as high-throughput quality check of tissues used for transplantation medicine.(MeSH Terms)
Ichimura, Taro, Kakizuka, Taishi, Taniguchi, Yoshitsugu, Ejima, Satoshi, Sato, Yuki, Itano, Keiko, Seiriki, Kaoru, Hashimoto, Hitoshi, Sugawara, Ko, Itoga, Hiroya, Onami, Shuichi, Nagai, Takeharu (2024) Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Published in 2024/12/27
(Abstract) None
Ichimura, Taro, Kakizuka, Taishi, Sato, Yuki, Itano, Keiko, Seiriki, Kaoru, Hashimoto, Hitoshi, Itoga, Hiroya, Onami, Shuichi, Nagai, Takeharu (2024) Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Published in 2024/02/28
(Abstract) None