in vivo and ex vivo live imaging dataset of mouse testes in an article "Self-organization of spermatogenic wave coordinates sustained sperm production in the mouse testis".
Description
Spermatogenesis takes place in the testis and relies upon the ordered turnover of differentiating cells supplied from spermatogenic stem cells. This process shows hierarchical spatiotemporal patterning, comprising the spermatogenic cycle, wave, and descent of segmental order, reflecting the currently underexplored mechanisms of ordered tissue and organ-scale homeostasis. Here, using mice, we conducted high-resolution, wide-field, and long-term live imaging studies in vivo and ex vivo, combined with whole-organ mapping of differentiation stages. Such trans-scale measures demonstrate that the stereotypic pattern of local cell turnover is coordinated into characteristic phase waves that propagate along the seminiferous tubules, becoming further organized at the organ-scale of tubule loops. Mathematical modeling indicates that such phase wave ordering can emerge from the local coupling of autonomous oscillators generated from delayed feedback regulation between stem and differentiating cells mediated by retinoic acid signaling. These findings provide evidence of a self-organization mechanism underpinning organ-scale homeostasis and constant sperm production, independent of external cues.
Grant-in-Aid for Scientific Research KAKENHI from MEXT and JSPS (JP25114004, JP15K21736, JP18H05551, JP 23H04952, and JP23H00380), Nanotechnology Platform Program <Molecule and Material Synthesis> of the MEXT (JPMXP09S16MS3009) , and AMED-CREST (JP22gm1110005h0006).
File formats
TIFF
Data size
1.1 TB
Organism
Mus musculus
Strain
C57BL/6J
Cell Line
NA
Genes
NA
Proteins
Stra8
GO Molecular Function (MF)
NA
GO Biological Process (BP)
biological phase, regulation of cell differentiation involved in tissue homeostasis, regulation of stem cell differentiation, retinoic acid receptor signaling pathway, regulation of male germ cell proliferation, germ cell development, tissue homeostasis, rhythmic process, spermatogenesis