This dataset contains the bright-field live cell images of murine immune-related cell lines (1008 cells) and primary murine peripheral blood mononuclear cells (PBMCs) (346 cells). Each cell has 30 frames that were captured in 29 min with 1 min interval. The cells of murine cell lines were determined as T cells (318 cells), leukemia cells (423 cells), or hematopoietic progenitor cells (267 cells) based on their subsequently measured whole transcriptomes. The PBMCs were determined as CD4+ T cells (152 cells), CD8+ T cells (103 cells), or B cells (91 cells) based on their subsequently measured whole transcriptomes. The determined cell types are indicated in an accompanying txt file.
Iamges were captured using an inverted optical microscope (Eclipse Ti2-E, Nikon) with an 20X objective lens (CFI Plan Apochromat Lambda 20X, Nikon), an LED (TI2-D-LHLED, Nikon), and a CMOS camera (Zyla5.5, Andor).