Neurogenic tagging assigns Cre-loxP recombination to newly differentiating neurons that share the neuronal birthdate by administration of tamoxifen (TM). It allows classification of neurons based on their birthdates and enables manipulation of the classified neurons in living animals. We have developed four neurogenic tagging driver mouse lines: Neurog2CreER(G2A), Neurog1CreER(G1C), Neurod1CreER(D1B) and Neurod4CreER(D4A). Depending on the line, neurons in different brain regions can be genetically tagged.
The NeuroGT database, also known as Brain Atlas of Neurogenic Tagging Mouse Lines, provides a catalog of image data of the neurons tagged by individual lines across the brain. In each dataset, tamoxifen (TM) is administrated at a single embryonic day (TM stage) throughout the neurodevelopmental period (E9.5~E18.5). The neurogenic-tagged neurons are stained either for their nuclei with a nuclear localized beta-galactosidase (nls-βGAL) reporter or whole their processes with a membrane-bound GFP (mGFP) reporter. We hope that this database will help people to find neurogenic tagging driver lines and the information useful for their research.