SSBD:database

Project ID

474-Yoshinari-NucleiAutofluoNIR

Project SID

474

Title

Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei

Description

Capturing images of the nuclear dynamics within live cells is an essential technique for comprehending the intricate biological processes inherent to plant cell nuclei. While various methods exist for imaging nuclei, including combining fluorescent proteins and dyes with microscopy, there is a dearth of commercially available dyes for live-cell imaging. In Arabidopsis thaliana, we discovered that nuclei emit autofluorescence in the near-infrared (NIR) range of the spectrum and devised a non-invasive technique for the visualization of live cell nuclei using this inherent NIR autofluorescence. Our studies demonstrated the capability of the NIR imaging technique to visualize the dynamic behavior of nuclei within primary roots, root hairs, and pollen tubes, which are tissues that harbor a limited number of other organelles displaying autofluorescence. We further demonstrated the applicability of NIR autofluorescence imaging in various other tissues by incorporating fluorescence lifetime imaging techniques. Nuclear autofluorescence was also detected across a wide range of plant species, enabling analyses without the need for transformation. The nuclear autofluorescence in the NIR wavelength range was not observed in animal or yeast cells. Genetic analysis revealed that this autofluorescence was caused by the phytochrome protein. Our studies demonstrated that nuclear autofluorescence imaging can be effectively employed not only in model plants but also for studying nuclei in non-model plant species.

Submission Date

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Opened Date

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Release Date

2026-06-09

Update Date

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Kind

License

Project URL

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Project DOI

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Metadata Version

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Template Version

3.0.2

Funding Information

This research was supported by the Japan Society for the Promotion of Science (JSPS) through grants JP20K21424 and JP23H02473 to M.N., JP18KK0195 to M.N. and A.Y., JP22K15139 to A.Y., JP23K05752 to N.Y., JP22H04926 and JP21K19256 to Y.S., and JP22H00360 to W.B.F.; by the Suntory Foundation of Life Sciences through a SUNBOR grant to M.N.; and a JST PRESTO grant, JPMJPR22D9, to A.Y. The ITbM is supported by the World Premier International Research Center Initiative (WPI), Japan.

Total

14 files / 3.0 GB

Image datasets

zipped 7 files / 453.8 MB, raw image 7 files / 2.6 GB, total 14 files / 3.0 GB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism

Strain

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Cell

Cell line

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MeSH

Anatomical Entity

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UBERON

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Biological Process

Cellular Component

Molecular Function

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Medical Subject Headings

Method involved in biological imaging