378-Minegishi-MetastaticNiche
Author reviewedFluorescent labeling of cell-cell interaction using sGRAPHIC
Project ID
378-Minegishi-MetastaticNiche
Project SID
378
Title
Fluorescent labeling of cell-cell interaction using sGRAPHIC
Description
Cancer cells inevitably interact with neighboring host tissue-resident cells during the process of metastatic colonization, establishing a metastatic niche to fuel their survival, growth, and invasion. However, the underlying mechanisms in the metastatic niche are yet to be fully elucidated owing to the lack of methodologies for comprehensively studying the mechanisms of cell–cell interactions in the niche. The authors improve a split green fluorescent protein (GFP)-based genetically encoded system to develop secretory glycosylphosphatidylinositol-anchored reconstitution-activated proteins to highlight intercellular connections (sGRAPHIC) for efficient fluorescent labeling of metastatic niche between tissue-resident cells and cancer cells.
Submission Date
2024-09-03
Opened Date
-
Release Date
2024-12-23
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
This research was supported by the Japan Society for Promotion of Science (KAKENHI grant no. JP17H04989, JP19H04814, JP20H02862 to T.Ku., JP21K18194 to H.S. and T.Ku., JP20J15062, JP22J00672 to M.M.), the CREST program of the Japan Agency for Medical Research and Development (grant no. JPMJCR2124 to H.S. and T.Ku.), the PRIME program of the Japan Agency for Medical Research and Development (grant no. JP20gm6210028 to N.T.), the Vehicle Racing Commemorative Foundation (to T.Ku, T.I. and N.T.), Takeda Science Foundation (to T.Ku.), and Yoshida Scholarship Foundation (to M.M.).
Total
1626 files / 1.4 GB
Image datasets
zipped 3 files / 211.5 MB, raw image 1623 files / 1.2 GB, total 1626 files / 1.4 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
-
Cell
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Cell line
EO771, NIH-3T3 cell
(
CLO:0008172
)
MC-38 cell, NIH-3T3 cell
(
CLO:0008172
)
MC-38 cell, AML12 cell
(
CLO:0001716
)
MeSH
NIH 3T3 Cells
(
D018613, D059008
)
Cell Communication
(
D018613, D059008
)
Ontology
Anatomical Entity
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UBERON
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Biological Process
cell-cell interaction
Cellular Component
cell-cell contact zone
(
GO:0044291
)
Molecular Function
-
Medical Subject Headings
NIH 3T3 Cells
(
D018613, D059008
)
Cell Communication
(
D018613, D059008
)
Imaging Method
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
time lapse microscopy
(
FBbi:00000249
)
Paper DOI / Paper URL
People
Contact
Takahiro Kuchimaru
Jichi Medical University
Graduate School of Medicine
-
-
Hirofumi Shintaku
RIKEN
Cluster for Pioneering Research
-
-
Imaging dataset contributor
Misa Minegishi (Tokyo Institute of Technology)
Tomomi Shimogori (RIKEN)
Atsushi Miyawaki (RIKEN)
Hiroshi Hamana (University of Toyama)
Hiroyuki Kishi (University of Toyama)
Quantitative dataset contributor
-
Image datasets
3
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time lapse images visualizing interactions between fibroblasts and breast cancer cells
Time lapse images visualizing interactions between fibroblasts and colorectal cancer cells
Time lapse images visualizing interactions between hepatocytes and colorectal cancer cells