393-Fujimoto-CoV2Infect/Fig5E_VB_tofacitinib
Author reviewedZ-series images of actin filaments in VB in SARS-CoV-2-infected devices with the administration of tofacitinib
Project
Dataset ID
Fig5E_VB_tofacitinib
Kind
Image data
Title
Z-series images of actin filaments in VB in SARS-CoV-2-infected devices with the administration of tofacitinib
Description
Z-series images of actin filaments in vascular bed (VB) in SARS-CoV-2-infected devices with the administration of tofacitinib, a JAK/STAT inhibitor. In the 3D culture device, vascular bed and bronchial organoids were co-cultured, and the vitrus was infected to the bronchial organoids. The inhibitor was applied to the VB existing channel.
Channel1: DAPI, Channel2: Alexa Fluor 647 labeled phalloidin Channel3: GFP, Channel4: ICAM1, Channel5: Differential interference image
Folder Name
Fig5E_VB_tofacitinib
File Format
.tif
File Size
48MB
File Separation Method
-
Figure Number
Fig5E
Dimensions
600x400x20x5x1
X Scale
1.24 micrometer
Y Scale
1.24 micrometer
Z Scale
3.22 micrometer
T Scale
-
Channel
5 channel
Quantitative Data
-
Biosample
Description
Co-culture of bronchial organoids, which were derived from normal human bronchial epithelial
cells, and vascular beds, which were composed from GFP-Expressing Human Umbilical Vein Endothelial Cells, in a special microfluidic device.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
endothelial cell of umbilical vein,
bronchial epithelial cell (CL:0002328)
Cell line
-
MeSH
Intrinsic Variable
The commercial HUVECSs expressing GFP were used.
GFP-HUVECs
(Angio-Proteomie, cAP-0001, Boston, MA, USA)
Extrinsic Variable
The SARS-CoV-2 virus was applied to the bronchial organoids,
Tofacitinib was applied to the vascular bed channel.
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
viral process (GO:0016032)
Cellular Component
nucleus (GO:0005634)
actin cytoskeleton (GO:0015629)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Gene
-
Protein / Probe
Intercellular adhesion molecule 1 / antibody
Method / Genotype
-
Gene
-
Protein / Probe
Actin, cytoplasmic 1 / affinity for specific proteins,
phalloidin
Method / Genotype
-
Gene
-
Protein / Probe
-DAPI
Method / Genotype
-
Treatments
Reagent / Compound
SARS-CoV-2 virus
Concentration
0.1 multiplicity of infection
Fold Dilution
-
Reagent / Compound
tofacitinib
Concentration
1 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody,
GFP,
4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
MeSH
Instrument
Body
Olympus-Evident FV3000
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident UPLXAPO10X
Filter Set
-
Dichroic
-