393-Fujimoto-CoV2Infect/Fig5A_KO-VB_SARS
Author reviewedZ-series images of KO-VB in the SARS-CoV-2-infected device
Project
Dataset ID
Fig5A_KO-VB_SARS
Kind
Image data
Title
Z-series images of KO-VB in the SARS-CoV-2-infected device
Description
Z-series images of KO-VB in the SARS-CoV-2-infected device in which KO-VB and bronchial organoids were co-culured. KO-VB is vascular bed made from IFNAR2 KO (Knocking out interferon alpha and beta receptor subunit 2)-HUVECs.
Channel1: CD31, Channel2: Alexa Fluor 647 labeled phalloidin, Channel3: DAPI, Channel4: Differential interference image
Folder Name
Fig5A_KO-VB_SARS
File Format
.oir
File Size
928MB
File Separation Method
-
Figure Number
Fig5A
Dimensions
2048x2048x29x4x1
X Scale
0.31 micrometer
Y Scale
0.31 micrometer/pixel
Z Scale
2.88 micrometer
T Scale
-
Channel
4 channel
Quantitative Data
-
Biosample
Description
Co-culture of bronchial organoids, which were derived from normal human bronchial epithelial
cells, and vascular beds, which were composed from IFNAR2 KO (Knocking out interferon alpha and beta receptor subunit 2)-HUVECs, in a special microfluidic device.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
endothelial cell of umbilical vein,
bronchial epithelial cell (CL:0002328)
Cell line
-
MeSH
Intrinsic Variable
Interferon alpha and beta receptor subunit 2 were knocked out from Human Umbilical Vein Endothelial Cells.
Extrinsic Variable
The SARS-CoV-2 virus was applied to the bronchial organoids,
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
viral process (GO:0016032)
Cellular Component
nucleus (GO:0005634)
actin cytoskeleton (GO:0015629)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
Platelet endothelial cell adhesion molecule / antibody
Method / Genotype
-
Gene
-
Protein / Probe
Actin, cytoplasmic 1 / affinity for specific proteins,
phalloidin
Method / Genotype
-
Gene
-
Protein / Probe
-DAPI
Method / Genotype
-
Gene
IFNAR2
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
SARS-CoV-2 virus
Concentration
0.1 multiplicity of infection
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody,
GFP,
4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
MeSH
Instrument
Body
Olympus-Evident FV3000
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident LUCPLFLN20X
Filter Set
-
Dichroic
-