393-Fujimoto-CoV2Infect/Fig4A_VB_Before-infect
Author reviewedFluorescent images of the vascular bed before infection with mock virus or SARS-CoV-2 virus
Project
Dataset ID
Fig4A_VB_Before-infect
Kind
Image data
Title
Fluorescent images of the vascular bed before infection with mock virus or SARS-CoV-2 virus
Description
Fluorescent images of the vascular bed 4-days before infection with mock virus or SARS-CoV-2 virus. Bronchial organoids and vascular bed composed of HUVECs (Human Umbilical Vein Endothelial Cells) were co-cultured in a special 3D microfluidic device. GFP-expressing HUVECs were used to observe the morphology of vascular bed. Twenty independent images are deposited.
Folder Name
Fig4A_VB_Before-infect
File Format
.tif
File Size
142MB
File Separation Method
20 independent data
Figure Number
Fig4A
Dimensions
1920x1200x1x1x1
X Scale
0.93 micrometer
Y Scale
0.93 micrometer
Z Scale
-
T Scale
-
Channel
1 channel
Quantitative Data
-
Biosample
Description
Co-culture of bronchial organoids, which were derived from normal human bronchial epithelial
cells, and vascular beds, which were composed from GFP-Expressing Human Umbilical Vein Endothelial Cells, in a special microfluidic device.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
endothelial cell of umbilical vein,
bronchial epithelial cell (CL:0002328)
Cell line
-
MeSH
Intrinsic Variable
The commercial HUVECSs expressing GFP were used.
GFP-HUVECs
(Angio-Proteomie, cAP-0001, Boston, MA, USA)
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
-
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
fluorescence microscopy (FBbi:00000246)
Detection Method
-
Visualization Method
Green fluorescent protein from Aequorea
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident CKX53
Module
-
Light Source
U-RFL-T
Detector
Olympus-Evident DP74
Objective
Olympus-Evident CACHN10XIPC
Filter Set
U-FBNA
Dichroic
-