393-Fujimoto-CoV2Infect/Fig4A_VB_After-infect_SARS
Author reviewedFluorescent images of the vascular bed 4days-after infection with SARS-CoV-2 virus
Project
Dataset ID
Fig4A_VB_After-infect_SARS
Kind
Image data
Title
Fluorescent images of the vascular bed 4days-after infection with SARS-CoV-2 virus
Description
Fluorescent images of the vascular bed 4-days after infection with SARS-CoV-2 virus. Bronchial organoids and vascular bed composed of HUVECs (Human Umbilical Vein Endothelial Cells) were co-cultured in a special 3D microfluidic device. GFP-expressing HUVECs were used to observe the morphology of vascular bed. The virus was applied to the side of bronchial organoids, but not to the vascular bed side. Nine independent images are deposited.
Folder Name
Fig4A_VB_After-infect_SARS
File Format
.tif
File Size
64MB
File Separation Method
9 independent data
Figure Number
Fig4A
Dimensions
1920x1200x1x1x1
X Scale
0.47 micrometer
Y Scale
0.47 micrometer
Z Scale
-
T Scale
-
Channel
1 channel
Quantitative Data
-
Biosample
Description
Co-culture of bronchial organoids, which were derived from normal human bronchial epithelial
cells, and vascular beds, which were composed from GFP-Expressing Human Umbilical Vein Endothelial Cells, in a special microfluidic device.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
endothelial cell of umbilical vein,
bronchial epithelial cell (CL:0002328)
Cell line
-
MeSH
Human Umbilical Vein Endothelial Cells (D061307)
Microscopy, Fluorescence (D008856)
COVID-19 (D000086382)
Epithelial Cells (D004847)
Intrinsic Variable
The commercial HUVECSs expressing GFP were used.
GFP-HUVECs
(Angio-Proteomie, cAP-0001, Boston, MA, USA)
Extrinsic Variable
The SARS-CoV-2 virus was applied to the bronchial organoids,
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
viral process (GO:0016032)
Cellular Component
-
Molecular Function
-
MeSH Anatomy
Human Umbilical Vein Endothelial Cells (D061307)
Microscopy, Fluorescence (D008856)
COVID-19 (D000086382)
Epithelial Cells (D004847)
MeSH BP
Human Umbilical Vein Endothelial Cells (D061307)
Microscopy, Fluorescence (D008856)
COVID-19 (D000086382)
Epithelial Cells (D004847)
MeSH CC
Human Umbilical Vein Endothelial Cells (D061307)
Microscopy, Fluorescence (D008856)
COVID-19 (D000086382)
Epithelial Cells (D004847)
MeSH MF
Human Umbilical Vein Endothelial Cells (D061307)
Microscopy, Fluorescence (D008856)
COVID-19 (D000086382)
Epithelial Cells (D004847)
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
SARS-CoV-2 virus
Concentration
0.1 multiplicity of infection
Fold Dilution
-
Imaging Method
Imaging Method
fluorescence microscopy (FBbi:00000246)
Detection Method
-
Visualization Method
Green fluorescent protein from Aequorea
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method
MeSH
Human Umbilical Vein Endothelial Cells (D061307)
Microscopy, Fluorescence (D008856)
COVID-19 (D000086382)
Epithelial Cells (D004847)
Instrument
Body
Olympus-Evident CKX53
Module
-
Light Source
U-RFL-T
Detector
Olympus-Evident DP74
Objective
Olympus-Evident CACHN20XIPC
Filter Set
U-FBNA
Dichroic
-