377-Ichinose-RedAlgae/Fig4_CellDiv
Author reviewedTime-lapse images of Cyanidioschyzon merolae cells stably expressing beta-tubulin-sfGFP
Project
Dataset ID
Fig4_CellDiv
Kind
Image data
Title
Time-lapse images of Cyanidioschyzon merolae cells stably expressing beta-tubulin-sfGFP
Description
Time-lapse images of red algae (Cyanidioschyzon merolae) cells stably expressing beta-tubulin-sfGFP. Beta-tubulin change its distribution during a certain period of the cell cycle. After cell division, beta-tubulin-sfGFP is observed in the daughter cells for 90 min and then disappeared during the G1 phase.. Channel1; sfGFP, Channel2 ; auto-fluorescence of chlorophyll, Channel3; bright field
Folder Name
Fig4_CellDiv
File Format
.tif
File Size
0.97GB
File Separation Method
-
Figure Number
Fig4ab
Dimensions
1024x1024x1x3x155
X Scale
1 micrometer
Y Scale
1 micrometer
Z Scale
-
T Scale
15 minutes
Channel
3 channel
Quantitative Data
-
Biosample
Description
The uracil-auxotrophic mutant (T1) of Cyanidioschyzon merolae stably expressing bata- tubulin-sfGFP. Superfolder GFP (sfGFP), a GFP with high thermal stability is suitable for culture condition of C. merolae at 42 °C.
Organism
Cyanidioschyzon merolae (NCBITaxon:45157)
Strain
Cell
red algae (Rhodophyta) (NCBITaxon:2763)
Cell line
-
Intrinsic Variable
The mutant Cyanidioschyzon merolae, T1, in which the URA5.3 gene is completely deleted,
Extrinsic Variable
The cells were stabely expressed superfolder GFP (sfGFP), a GFP with high thermal stability
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
cell cycle (GO:0007049) (GO:0007049)
cell division (GO:0051301)
Cellular Component
-
Molecular Function
tubulin binding (GO:0015631)
Genetics
Gene
CMN263C
Protein / Probe
Tubulin beta chain
Method / Genotype
insertion
Gene
URA5.3
Protein / Probe
-
Method / Genotype
deletion
Treatments
No treatment records.
Imaging Method
Imaging Method
fluorescence microscopy (FBbi:00000246)
Detection Method
wide-field detection
Visualization Method
fluorescent protein tag, autofluorescence
Illumination Method
widefield illumination
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
dispersed cells in vitro
Instrument
Body
OLYMPUS FV3000
Module
-
Light Source
-
Detector
-
Objective
USLSAPO 100XS (OLYMPUS)
Filter Set
-
Dichroic
-